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Goserelin (peptide) from Serum using Microelution SPE and LC/MS/MS Analysis
Peptides have been known to display poor recoveries when subjected to dry down steps due to their extremely hydrophobic nature. By using a microelution SPE sample preparation method, we were able to skip the dry down step which saved at least 30 minutes and also helped to avoid loss of the peptide. Goserelin was analyzed by LC/MS/MS in under 3 minutes and was accurately quantified at 5 ng/mL.
Goserelin (peptide) from Serum using Microelution SPE and LC/MS/MS Analysis
Peptides have been known to display poor recoveries when subjected to dry down steps due to their extremely hydrophobic nature. By using a microelution SPE sample preparation method, we were able to skip the dry down step which saved at least 30 minutes and also helped to avoid loss of the peptide. Goserelin was analyzed by LC/MS/MS in under 3 minutes and was accurately quantified at 5 ng/mL.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase, Solid Phase Extraction
Phases:
Goserelin (peptide) from Serum using Microelution SPE and LC/MS/MS Analysis
Peptides have been known to display poor recoveries when subjected to dry down steps due to their extremely hydrophobic nature. By using a microelution SPE sample preparation method, we were able to skip the dry down step which saved at least 30 minutes and also helped to avoid loss of the peptide. Goserelin was analyzed by LC/MS/MS in under 3 minutes and was accurately quantified at 5 ng/mL.
| Target Industries: | Biopharmaceutical Analysis, Pharmaceutical |
| Techniques: | HPLC, HPLC Reversed Phase, Sample Preparation, Solid Phase Extraction |
| Brands: | Aeris HPLC Column |
| Separation Modes: | Reversed Phase, Solid Phase Extraction |
| Phases: | Aeris Widepore XB-C18, Strata-X-CW |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.