Goserelin (peptide) from Serum using Microelution SPE and LC/MS/MS Analysis

Peptides have been known to display poor recoveries when subjected to dry down steps due to their extremely hydrophobic nature. By using a microelution SPE sample preparation method, we were able to skip the dry down step which saved at least 30 minutes and also helped to avoid loss of the peptide. Goserelin was analyzed by LC/MS/MS in under 3 minutes and was accurately quantified at 5 ng/mL.

Document Type:

Technical Notes

Target Industries:

Biopharmaceutical Analysis, Pharmaceutical

Techniques:

HPLC, HPLC Reversed Phase, Sample Preparation, Solid Phase Extraction

Brands:

Aeris

Separation Modes:

Reversed Phase, Solid Phase Extraction

Phases:

Aeris Widepore XB-C18, Strata-X-CW