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Investigating the effect of column ID on sensitivity when using Micro LC columns

In this application note, we investigate the ability to scale separations developed on traditional analytical columns (2.1 mm ID) to micro flow rate scale columns (0.3 and 0.5 mm ID) to take advantage of the sensitivity gains through miniaturization without the need for new method development and optimization. The comparison was generated using a representative sample of 20 stable-isotope-labelled (SIL) peptides under typical reversed-phase mobile phase conditions using a SCIEX 5500 QTRAP MS/MS detector. This application demonstrates the effect on sensitivity and selectivity when column ID is reduced into a micro LC format.