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Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, room temperature hydrolysis efficiency using IMCSzyme RT enzyme was studied by analyzing fortified, pooled human urine samples with a Kinetex biphenyl LC column and LC-MS/MS quantitation. Next. 96 authentic human urine samples were prepared using a "dilute and shoot" method and extracted using Beta-Gone Plus with in-well hydrolysis. Results generated using both sample preparation methods agreed, but the samples prepared using Beta-Gone Plus produced a time savings of 45 minutes per batch over the dilute and shoot method, and the cleaner Beta-Gone samples resulted in longer LC column lifetime.
Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, room temperature hydrolysis efficiency using IMCSzyme RT enzyme was studied by analyzing fortified, pooled human urine samples with a Kinetex biphenyl LC column and LC-MS/MS quantitation. Next. 96 authentic human urine samples were prepared using a "dilute and shoot" method and extracted using Beta-Gone Plus with in-well hydrolysis. Results generated using both sample preparation methods agreed, but the samples prepared using Beta-Gone Plus produced a time savings of 45 minutes per batch over the dilute and shoot method, and the cleaner Beta-Gone samples resulted in longer LC column lifetime.
Document Type:
Technical Notes
Target Industries:
Separation Modes:
Beta-Glucuronidase Removal, Reversed Phase, Solid Phase Extraction
Phases:
Fast, Simple Urine Drug Extraction with In-well Room Temperature Hydrolysis and Enzyme Removal to Extend LC Column Life
In this technical note, room temperature hydrolysis efficiency using IMCSzyme RT enzyme was studied by analyzing fortified, pooled human urine samples with a Kinetex biphenyl LC column and LC-MS/MS quantitation. Next. 96 authentic human urine samples were prepared using a "dilute and shoot" method and extracted using Beta-Gone Plus with in-well hydrolysis. Results generated using both sample preparation methods agreed, but the samples prepared using Beta-Gone Plus produced a time savings of 45 minutes per batch over the dilute and shoot method, and the cleaner Beta-Gone samples resulted in longer LC column lifetime.
| Target Industries: | Clinical Research, Forensic Toxicology |
| Techniques: | Beta-Glucuronidase, HPLC, HPLC Reversed Phase, Sample Preparation, Solid Phase Extraction |
| Brands: | Beta-Gone, Kinetex |
| Separation Modes: | Beta-Glucuronidase Removal, Reversed Phase, Solid Phase Extraction |
| Phases: | Kinetex Biphenyl |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.