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Aggregate Analysis of Tirzepatide Using a Biozen dSEC-1 SEC Column
This study highlights the performance of Biozen dSEC-1 SEC columns for detecting and quantifying aggregates in Tirzepatide, a large hydrophobic peptide prone to aggregation. Both 150 mm (1.6 um) and 300 mm (3 um) columns achieved reliable separation of monomer and aggregate with excellent reproducibility (retention time RSD ≤ 0.08%, peak area RSD ≤ 4.35%) and strong sensitivity (S/N > 7). Notably, this was accomplished using a standard buffer with only moderate organic content, demonstrating the column's highly hydrophilic stationary phase and suitability for robust, reproducible aggregation analysis without harsh mobile phase conditions.
Aggregate Analysis of Tirzepatide Using a Biozen dSEC-1 SEC Column
This study highlights the performance of Biozen dSEC-1 SEC columns for detecting and quantifying aggregates in Tirzepatide, a large hydrophobic peptide prone to aggregation. Both 150 mm (1.6 um) and 300 mm (3 um) columns achieved reliable separation of monomer and aggregate with excellent reproducibility (retention time RSD ≤ 0.08%, peak area RSD ≤ 4.35%) and strong sensitivity (S/N > 7). Notably, this was accomplished using a standard buffer with only moderate organic content, demonstrating the column's highly hydrophilic stationary phase and suitability for robust, reproducible aggregation analysis without harsh mobile phase conditions.
Document Type:
Technical Notes
Target Industries:
Brands:
Separation Modes:
Size Exclusion
Phases:
Aggregate Analysis of Tirzepatide Using a Biozen dSEC-1 SEC Column
This study highlights the performance of Biozen dSEC-1 SEC columns for detecting and quantifying aggregates in Tirzepatide, a large hydrophobic peptide prone to aggregation. Both 150 mm (1.6 um) and 300 mm (3 um) columns achieved reliable separation of monomer and aggregate with excellent reproducibility (retention time RSD ≤ 0.08%, peak area RSD ≤ 4.35%) and strong sensitivity (S/N > 7). Notably, this was accomplished using a standard buffer with only moderate organic content, demonstrating the column's highly hydrophilic stationary phase and suitability for robust, reproducible aggregation analysis without harsh mobile phase conditions.
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC, HPLC-SEC |
| Brands: | Biozen LC |
| Separation Modes: | Size Exclusion |
| Phases: | Biozen dSEC-1 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.