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Optimized Solid-Phase Extraction of GalNAc-Conjugated siRNA from Plasma and Liver Matrices Using Clarity OTX Pro
GalNAc-conjugated siRNA therapeutics present significant bioanalytical challenges due to their low dosing, complex structures, and strong matrix interactions. This technical note describes an optimized solid-phase extraction (SPE) workflow using Clarity OTX Pro for sensitive and reproducible quantitation of Inclisiran in plasma and liver S9 fraction. High recoveries and excellent precision were achieved for both antisense and sense strands in plasma, with low limits of quantitation. In liver S9 fraction, Proteinase K pre-treatment improved extraction efficiency, while use of a 2 mg/well micro-elution format delivered substantial gains in sensitivity, including a 10-fold lower LLOQ for the antisense strand. Together, these results demonstrate a robust, flexible SPE strategy for reliable bioanalysis of GalNAc-siRNA therapeutics across complex biological matrices.
Optimized Solid-Phase Extraction of GalNAc-Conjugated siRNA from Plasma and Liver Matrices Using Clarity OTX Pro
GalNAc-conjugated siRNA therapeutics present significant bioanalytical challenges due to their low dosing, complex structures, and strong matrix interactions. This technical note describes an optimized solid-phase extraction (SPE) workflow using Clarity OTX Pro for sensitive and reproducible quantitation of Inclisiran in plasma and liver S9 fraction. High recoveries and excellent precision were achieved for both antisense and sense strands in plasma, with low limits of quantitation. In liver S9 fraction, Proteinase K pre-treatment improved extraction efficiency, while use of a 2 mg/well micro-elution format delivered substantial gains in sensitivity, including a 10-fold lower LLOQ for the antisense strand. Together, these results demonstrate a robust, flexible SPE strategy for reliable bioanalysis of GalNAc-siRNA therapeutics across complex biological matrices.
Document Type:
Technical Notes
Target Industries:
Brands:
Separation Modes:
Reversed Phase, Solid Phase Extraction
Phases:
Optimized Solid-Phase Extraction of GalNAc-Conjugated siRNA from Plasma and Liver Matrices Using Clarity OTX Pro
GalNAc-conjugated siRNA therapeutics present significant bioanalytical challenges due to their low dosing, complex structures, and strong matrix interactions. This technical note describes an optimized solid-phase extraction (SPE) workflow using Clarity OTX Pro for sensitive and reproducible quantitation of Inclisiran in plasma and liver S9 fraction. High recoveries and excellent precision were achieved for both antisense and sense strands in plasma, with low limits of quantitation. In liver S9 fraction, Proteinase K pre-treatment improved extraction efficiency, while use of a 2 mg/well micro-elution format delivered substantial gains in sensitivity, including a 10-fold lower LLOQ for the antisense strand. Together, these results demonstrate a robust, flexible SPE strategy for reliable bioanalysis of GalNAc-siRNA therapeutics across complex biological matrices.
| Target Industries: | Biopharmaceutical Analysis |
| Techniques: | HPLC, HPLC Reversed Phase, Oligonucleotide Analysis, Overview, Solid Phase Extraction |
| Brands: | ClaritySPE |
| Separation Modes: | Reversed Phase, Solid Phase Extraction |
| Phases: | Clarity OTX Pro |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.