|Industry Applications|Pharmaceutical
HPLC
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kinetex-hplc-column
aeris-peptide-xb-c18

Pharmaceutical

From Sample Preparation to Analysis, We Have You Covered

The analysis of pharmaceutical molecules provides challenges which we aim to help you overcome. From sample preparation of PK/ADME samples, to complex HPLC/UHPLC separations and residual solvent analysis we have the solutions you need. We provide:

  • Method development/pharma workflow support,
  • Troubleshooting for sample preparation, HPLC, and GC
  • Cost effective analytical solutions.

Discover a variety of pharmaceutical solutions tailored to you. You can also easily access the Nitrosamines and NDSRI application portal.

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Therapeutic Peptides

Therapeutic peptides are short chains of amino acids that have emerged as a promising class of drugs due to their high specificity, potency, and favorable safety profiles. They mimic natural peptides in the body, allowing them to engage with specific receptors and pathways involved in various physiological processes. This makes them particularly effective in treating a wide range of conditions, including metabolic disorders, cancer, and infectious diseases. Their small size and ability to be easily modified for improved stability and delivery further enhance their therapeutic potential. As a result, therapeutic peptides are becoming increasingly important in the development of novel treatments with fewer side effects compared to traditional small molecule drugs.

High-Performance Liquid Chromatography (HPLC) is a vital technique for purifying therapeutic peptides, ensuring their efficacy and safety. By exploiting differences in peptide properties such as size, charge, and hydrophobicity, HPLC effectively separates and isolates the desired peptide from impurities and by-products. The method's high resolution and precision make it ideal for producing peptides with high purity, which is crucial for clinical applications. Additionally, HPLC can be scaled up for industrial production, providing a reliable and consistent means of purification. This ensures that therapeutic peptides meet stringent regulatory standards, ultimately leading to more effective and safer treatments for patients.

GLP-1 (Glucagon-Like Peptide-1) analogues are a specific focus, with their potential to regulate blood sugar levels, slowing gastric emptying and promoting a feeling of fullness. Several drugs are already on the market (semaglutide, dulaglutide, exenatide and liraglutide) and finding successful strategies for the purification and quality assurance of these therapeutic peptides is of current interest to many pharmaceutical companies.

4
Optimized HPLC Method for Assay and Separation of Semaglutide from its Known Impurities/Related Substances using Aeris Peptide XB-C18
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Stability Indicating HPLC Method for the Determination of Semaglutide Related Substances and Degradation Products Using Aeris Peptide XB-C18
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/documents/stability-indicating-hplc-method-for-the-determination-of-semaglutide-related-substances-and-degrada
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Glucagon-Like Peptide-1 (GLP-1) Analogues Applications Notebook
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/documents/glucagonlike-peptide1-glp1-analogues-applications-notebook
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Change Selectivity, Not the Stationary Phase, for Synthetic Peptide Multi-Step Purifications
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/documents/change-selectivity-not-the-stationary-phase-for-synthetic-peptide-multistep-purifications-tn1177
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More Accurate Purity Assessments of Peptide Process Purifications Using Kinetex Core-Shell Media
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/documents/more-accurate-purity-assessments-of-peptide-process-purifications-using-kinetex-coreshell-media-tn10
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Stationary Phases for the Process Scale Purification of Peptides and Insulin Analogs
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/documents/stationary-phases-for-the-process-scale-purification-of-peptides-and-insulin-analogs-tn1233
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Aeris Peptide XB-C18 Core-Shell HPLC Column
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Axia
Longest Lasting Preparative HPLC and SFC ColumnLongest Lasting Preparative HPLC and SFC Column
/products/axia-preparative-lc-column
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Biozen dSEC-2
Size Exclusion Chromatography
/products/biozenlc-hplc-column/biozen-dsec
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Biozen Peptide
Size Exclusion Chromatography
/products/biozenlc-hplc-column
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Kinetex
Core-Shell Performance on Any LC System
/products/kinetex-hplc-column
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Luna
One of the World’s Leading LC columns
/products/luna-hplc-column
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Webinars

/resources/webinars/preparative-chromatography-of-peptides-developing-twostep-purification-processes-on-a-single-station
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Preparative Chromatography of Peptides: Developing Two-step Purification Processes on a Single Stationary Phase
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Preparative Chromatography: Choosing the Right Strategy for Your Methodology
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21452,21453,21454

Applications

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Originator Pharmaceutical Drug Development

Our team of experts understand the challenges associated with developing methods to support small molecule Drug development and analysis. Let us help you to utilise our portfolio of HPLC and UHPLC products to your benefit during method development and validation.

4
Drug and Metabolites Guide
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/documents/drug-and-metabolites-guide
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Determination of Chloroquine, Hydroxychloroquine and its Metabolite Desethyl Hydroxychloroquine in Plasma Samples by LC-MS/MS
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/documents/determination-of-chloroquine-hydroxychloroquine-and-its-metabolite-desethyl-hydroxychloroquine-in-pl
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Comparison of Two High-Performance Particle Morphologies in the Separation of Hydrochlorothiazide and Chlorothiazide
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/documents/comparison-of-two-highperformance-particle-morphologies-in-the-separation-of-hydrochlorothiazide-and
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Forced Degradation of Cefaclor Analysis using Kinetex EVO C18 Core-Shell LC Column
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/documents/forced-degradation-of-cefaclor-analysis-using-kinetex-evo-c18-core-shell-lc-column
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Greater separation muscle and retention. Consistent pore structure and elimination of micropores. Extreme ruggedness and reproducibility.
/products/luna-omega-hplc-column
#F2F5F9
Core-shell performance gains. Supports method portability. Improves productivity.
/products/kinetex-hplc-column
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New Polymeric Sorbent with Matrix Removal Technology Provides Unparalleled Reproducibility High Recoveries without Conditioning or Equilibration.
/products/strata-x-pro-solid-phase-extraction-products
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Webinars

https://phxstage--phx-stage--phenomenex-it.aem.live/
true
Understanding Reversed Phase Selectivity for Different Compound Classes
false
26550,23727,22972

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Generic Pharmaceuticals

Bringing drugs to market in a quicker, more affordable manner poses significant challenges. We have solutions allowing you to reduce analysis time and cost, giving you a competitive advantage.

4
Kinetex Core-Shell LC Column Guide - Pharmaceutical Edition
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/documents/kinetex-core-shell-lc-column-guide---pharmaceutical-edition
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USP Allowable Adjustments
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/documents/usp-allowable-adjustments-guide
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Demystifying L/dp Ration When Working with Allowable Adjustments
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/documents/demystifying-ldp-ratio-when-working-with-allowable-adjustments
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Luna Omega Product Guide - Pharma Edition
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/documents/luna-omega-product-guide---pharma-edition
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4
Separation of Ezetimibe and its Organic Impurities per USP Monograph
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/documents/separation-of-ezetimibe-and-its-organic-impurities-per-usp-monograph
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Ph. Eur. Monograph 2217: Lamivudine Related Substances with Ph. Eur. Method Modernization
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/documents/ph-eur-monograph-2217-lamivudine-related-substances-with-ph-eur-method-modernization
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Greater separation muscle and retention. Consistent pore structure and elimination of micropores. Extreme ruggedness and reproducibility.
/products/luna-omega-hplc-column
#F2F5F9
Core-shell performance gains. Supports method portability. Improves productivity.
/products/kinetex-hplc-column
#F2F5F9
Versatile: NP, PO, RP and SFC stable. High efficiency and loadability. Robust: Pressure stable to 300 bar.
/products/lux-chiral-lc-column
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Webinars

/resources/webinars/harmonization-of-pheur-2246-and-usp-621-new-allowed-adjustments
true
Harmonization of Ph.Eur. 2.2.46 and USP 621 – New allowed adjustments
false
26294,23727,26338

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Active Pharmaceutical Ingredient Manufacturing

When developing your method we offer high resolution core-shell and thermally modified particles to provide you with high resolution separations to help you resolve related organic impurities from your API. We can provide support in method development, proposing suitable stationary phases in combination with mobile phases together with screening recommendations to save time and money. We also offer products which are scalable, for those separations where chromatographic purification is the only option.

For further information on our pre-packed preparative columns manufactured using AXIA technology, please click here. We also offer bulk material for DAC systems, further details can be found by following this link.

4
Acetylsalicylic Acid Impurities and Related Substances (EP Monograph 0309) by HPLC-UV
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/documents/acetylsalicylic-acid-impurities-and-related-substances-ep-monograph-0309-by-hplcuv-tn1071
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Easy Method Transfer from UHPLC to HPLC to PREP LC
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/documents/easy-method-transfer-from-uhplc-to-hplc-to-prep-lc-tn1135
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Scalability, Reproducibility, and Increased Separation Power of Luna Omega C18 (1.6 and 3 µm)
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/documents/importedfromlegacy/pdfdocuments/an40230621-w.pdf?rev=44db960fab8946b0a345c6fb1344cdca
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The Ultimate Guide to HPLC/UHPLC Reversed Phase Selectivity for API Manufacturers
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/documents/the-ultimate-guide-to-hplcuhplc-reversed-phase-selectivity-for-api-manufacturers
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4
Application Handbook, Chromatography Solutions for API Manufacturers
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/documents/application-handbook-chromatography-solutions-for-api-manufacturers
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Change Selectivity, Not the Stationary Phase, for Synthetic Peptide Multi-Step Purifications
View Document
/documents/change-selectivity-not-the-stationary-phase-for-synthetic-peptide-multistep-purifications-tn1177
#F2F5F9
Greater separation muscle and retention. Consistent pore structure and elimination of micropores. Extreme ruggedness and reproducibility.
/products/luna-omega-hplc-column
#F2F5F9
Core-shell performance gains. Supports method portability. Improves productivity.
/products/kinetex-hplc-column
#F2F5F9
High efficient preparative separations Increased bed density prevents bed collapse Durable preparative columns.
/products/axia-preparative-lc-column
#F2F5F9

Webinars

https://phxstage--phx-stage--phenomenex-it.aem.live/
true
HPLC troubleshooting and Column Care
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https://phxstage--phx-stage--phenomenex-it.aem.live/
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Understanding Reversed Phase Selectivity for Different Compound Classes
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Product Finder

Find the right products by selecting your pharmaceutical phase or workflow

Product Finder
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