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biozenspe-sample-preparation-products
biozen-n-glycan
Biozen N-Glycan Clean-Up
Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)
SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.
- Small volume format allows for more concentrated samples
- Minimal elution solvent
- Excellent retention and recovery of labeled, released N-glycans
- Faster clean-up methods to save on sample prep time
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Overview
Protocol
N-Glycan Clean-up
Reliable recovery and faster results
STEP 1
STEP 2
STEP 3
STEP 4
STEP 5
Start with Intact Glycoprotein
Deglycosylation
Released N-Glycans by
PNGase F in buffer solution
Labeling Glycans
2-AA or 2-AB for accurate and
sensitive glycan detection
Clean-Up
Removal of labeling
reagent, salts, or surfactants
Analysis
96-Well Plate:
Biozen N-Glycan Clean-up
Part No.:
8M-S009-NGA
Condition:
200 µL Water
Equilibrate:
200 µL Water/Acetonitrile (15:85)
Load:
Labeled Sample (up to 400 µL of labeled sample)
Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile
Biozen Columns (mm)
Biocompatible Guard Cartridges
50 x 2.1
100 x 2.1
150 x 2.1
/ 3pk
Labeled Glycans from AGP
Sum of Peak Areas for Z-value Calculation
Glycan
Sum of Peak Area (%)
Multiplication Factor
Charge Number Share
Asialo
0.28
0
0
Monosialo-
13.41
1
13.41
Disialo-
4.44
2
8.88
Trisialo-
52.49
3
157.47
Tetrasialo-
29.4
4
117.6
Total peak area
100%
Z-value= 297.36
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