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Biozen Biologics LC Columns

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Explore Biozen LC Portfolio
Elevate your protein analysis with precision and versatility using Biozen's advanced LC columns. Optimize your sensitive HPLC analysis for superior results.

Explore Biozen LC Portfolio

With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:

biozen-logo
Biozen bioTi 50 and 150

Biocompatible Flow Path with BioTi™ Hardware

Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!

Biozen Ti Technology

A: UHPLC Pressure Rating

Allows for more experimental design space; room to increase flow rates for increased throughput

B: Better Controlled ID Versus PEEK Hardware

Improved column-to-column performance, providing more consistency and easier method validation

C: Biocompatible Titanium Liner

D: Improved Recovery and Peak Shape

Better column-to-column consistency, ease of use, and troubleshooting

E: Strong Stainless Steel Walls Help Prevent Movement Under Pressure

F: Biocompatible Titanium Frit

4 Advanced Particle Platforms

All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.

Pore Controlled Technology

Pore Controlled Technology

Monosized Polymeric
Non-Porous
Monosized Polymeric Non-Porous

Core-Shell Technology

Core-Shell Technology

Thermally Modified
Fully Porous
Thermally ModifiedFully Porous

Pore Controlled Technology

The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.

Monosized Polymeric Non-Porous

Meticulously controlled monosized particle technology secures incredible particle consistency that leads to improved and reliable efficiency. This innovative non-porous particle serves as the perfect backbone for complex ion-exchange chemistries.

Core-Shell Technology

Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogeneous porous shell is grown on a solid silica core. This highly optimized process combined with industry leading column packing technology produces highly reproducible columns that generate extremely high efficiencies and sensitivity.

Thermally Modified Fully Porous

Through a proprietary thermal processing series of steps, we eliminate micropores and further improve consistency, column efficiency, inertness, ruggedness, and reproducibility.

11 Particle Chemistries

 

 

Oligonucleotide Analysis

The Biozen Oligo LC column brings a unique combination of improved chromatographic efficiency from its core-shell morphology and high pH stability necessary for oligonucleotide characterization. Biozen Oligo is packed in a unique bio-inert titanium hardware developed to mitigate non-specific interactions that lead to analyte loss, distortions in peak shape and carry over. Confidently maximize resolution and reproducibility throughout the most demanding of method extremes with Biozen Oligo.

LC-MS Analysis of siRNA using BioTi UHPLC Hardware

Bio-inert hardware gives clear separation of the sense and anti-sense strand. View application>>

Application ID 25911
LC columns

Columns:
Biozen 2.6 μm Oligo ( BioTi™)
Clarity 2.6 μm Oligo XT (stainless steel)
Dimensions
100 x 2.1 mm
Part No.:
Mobile Phase:
A: A: 4 mM Triethylamine in Water + 12.5 mM Hexafluoro 2 propanol
B: B: 4 mM Triethylamine in Methanol + 12.5 mM Hexafluoro 2 propanol

vs

LC-MS Analysis of siRNA using Stainless Steel UHPLC Hardware

Non-specific interactions were observed using stainless steel hardware resulting in bimodal peak for late eluting anti-sense strand.

Application 25911

Gradient:
Time (min)
0
2
16
16.1
20
20.1
 
% B
5
5
30
95
95
5
Flow Rate:
0.3 mL/min
Temperature:
55 °C
Instrument:
Shimadzu® LC 20A Prominence®
Detection:
TOF-MS
Detector:
SCIEX® TripleTOF ® 6600

Oligonucleotide Solid Phase Extraction (SPE)

The market leading oligonucleotide extraction solution for biotherapeutics. Effectively remove matrix interferences and achieve ultra-high recoveries through the use of a fast, simple 4-step protocol.

Clarity Well Plate

Peptide Mapping

Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed two Biozen Peptide columns to offer uniquely different selectivity profiles for these types of bioseparations. Each allows for fast and effective elution windows by utilizing either high efficiency core-shell or thermally modified fully porous particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.

Trastuzumab Biosimilar Peptide Map


Infliximab Biosimilar Peptide Map

Conditions for all columns:
Column:
  Biozen 1.6 µm Peptide PS-C18
  Biozen 2.6 µm Peptide XB-C18
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient:
Time (min)
0
0.5
50
55
56
 
% B
1
1
50
50
95
Flow Rate:
0.3 mL/min
Temperature:
40 °C
Detection:
QTOF (SCIEX®  X500B)
With protein aggregate often at very low levels (<0.1% by peak area compared to monomer) and fragment separation a requirement, adequate resolution and peak shape have become even more crucial method outcomes. To address this need, the robust set of Biozen SEC columns were developed with a combination of UHPLC efficiency and higher sensitivity, to drive resolution and identification of even lower level targets. Biozen dSEC-7 Brochure>>  Biozen dSEC-2 Brochure>>

27576
LC Conditions:

Column:
Biozen 3 µm dSEC-7
Dimensions
150 x 4.6 mm
Part No.:
Mobile Phase:
200 Potassium Phosphate +
250 mM KCl, pH 6.2
Part No.:
00H-4787-E0
Mobile Phase:
20 mM Sodium Phosphate, pH 6.6 + 350 mM Potassium Chloride
Flow Rate:
350 µL/min (Isocratic)
Injection Volume:
 legend_grn 0.5 µL
 legend_skyblue 1 µL
 legend_purple 2 µL
Temperature:
25 °C
Instrument:
Waters® ACQUITY® H-Class
Detection:
FLR - Ex 280 nm, Em 350 nm
Sampling Rate: 40 Hz
Sample:
1. AAV2-CAG-GFP, 2E13 vg/mL
2. AAV5-CMV-GFP, 2E13 vg/mL
3. AAV8-CMV-GFP, 2E13 vg/mL
4. AAV9-CMV-GFP, 2E13 vg/mL




26687
Conditions for all columns:

Column:
Biozen 1.8 µm dSEC-2, 200  Å
Dimensions
300 x 4.6 mm
Part No.:
Mobile Phase:
200 Potassium Phosphate +
250 mM KCl, pH 6.2
Part No.:
00H-4787-E0
Flow Rate:
0.35 mL/min
Injection Volume:
10 µL
Temperature:
25 °C
Detection:
UV @ 280 nm
Sample:
Various,10 mg/mL

Biozen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly tuned and controlled manufacturing process, Biozen WCX HPLC columns affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware. View Application Note>>

Trastuzumab (MES Salt Gradient)

Application ID 25058

Column:
Biozen 6 μm WCX
Dimensions
250 x 4.6 mm
Part No.:
Mobile Phase:
A: 20 mM MES (pH 5.6)
B: 20 mM MES + 300 mM NaCl (pH 5.6)
Gradient:
Time (min)
0
1
31
31.1
34
35
 
% B
15
15
45
100
100
15
Flow Rate:
1 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
Trastuzumab

Trastuzumab (pH Gradient Buffer)

Application ID 25061

Column:
Biozen 6 μm WCX
Dimensions
250 x 4.6 mm
Part No.:
Mobile Phase:
A: CX -1 (pH 5.6) pH Gradient Buffer*
B: CX -1 (pH 10.2) pH Gradient Buffer*
Gradient:
Time (min)
0
1
21
23
24
 
% B
0
0
100
100
0
Flow Rate:
1 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
Trastuzumab

* From Thermo Fisher Scientific® Inc.

Intact and Subunit Analysis

Impurity profiling and characterization of intact biologics and fragments is a challenging undertaking because of the need to identify very small differences between variants. Both Biozen Intact columns. contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target mAb HC/LC, Fc/Fab, or isoforms.

Intact Trastuzumab at 70, 80, and 90 °C

Conditions for all columns:

Column:
Biozen 3.6 µm Intact XB-C8
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient:
Time (min)
0
1
13
 
% B
20
20
25
Flow Rate:
0.5 mL/min
Temperature:
70 °C
80 °C
90 °C
Detection:
UV @ 280 nm
Sample:
1. Trastuzumab

Infliximab F(ab)2

Column:
Biozen 3.6 µm Intact XB-C8
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient:
Time (min)
0
1
13
 
% B
20
20
60
Flow Rate:
0.5 mL/min
Temperature:
80 °
Detection:
UV @ 280 nm
Sample:
1. Infliximab F(ab)2

Cetuximab

Column:
Biozen 3.6 µm Intact XB-C8
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient:
Time (min)
0
1
13
 
% B
20
20
45
Flow Rate:
0.5 mL/min
Temperature:
80 °
Detection:
UV @ 280 nm
Sample:
1. Cetuximab

MagBeads Streptavidin Coated

Higher binding capacity magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

bioti magbead

Intact Mass Analysis

Intact mass can give indications not only of relative abundance of glycoforms , but also stability as degraded mAbs will not give good charge envelope by ESI-MS. Intact Mass with a high resolution MS to identify PTMs, especially relative abundance of glycoforms , combines extremely well with the fast run times and tight peak shapes provided by the Biozen Intact XB-C8 and Biozen WidePore C4.

Intact Mass of Trastuzumab Biosimilar using a Biozen Intact XB-C8 and SCIEX® X500B

Application ID 25065 25067

Column:
Biozen 3.6 µm Intact XB-C8
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile / Isopropyl alcohol (50:50
Gradient:
Time (min)
2.5
10
110.1
 
% B2
0
65
95
Flow Rate:
0.3 mL/min
Temperature:
90 °
Detection:
QTOF (SCIEX X500B)
Sample:
Trastuzumab

Drug Antibody Ratio (DAR)

With a direct effect on efficacy and safety, conjugation for each ADC must be well understood. The Biozen Intact XB-C8 provides an excellent vehicle for determining drug load distribution and DAR for ADCs. Its large pore size allows intact ADCs to interact with a moderately retentive stationary phase while the core-shell particle supplies increased efficiency to deliver the required resolution between ADC species with differing drug loads.

Herceptin—vcMMAE using Biozen 3.6 µm Intact XB-C8

Herceptin—mcMMAF using Biozen 3.6 µm Intact XB-C8


Peptide Quantitation

When quantitating signature peptides from biological matrices, you need sharp peak shape and sufficient retention of hydrophilic peptides to prevent any signal loss from matrix suppression regions. Both Biozen Peptide columns were developed to deliver excellent selectivity for even closely related peptides. Additionally, they build on this body of valuable characteristics with unique ways of delivering sharper peak shape for basic peptides; Biozen Peptide XB-C18 blocks secondary surface interactions via isobutyl side chains, while the Biozen Peptide PS-C18 contains a positively charged weak base that repels other basic species.

Column:
Biozen 3 µm Peptide PS-C18
Dimensions
50 x 2.1 mm
Part No.:
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient:
Time (min)
0
1
4.5
 
%B
3
3
25
Flow Rate:
0.5 mL/min
Temperature:
22 °C
LC System:
ExionLC™ AD HPLC
Detection:
MS/MS
Detector:
SCIEX QTRAP® 5500
Flow Rate:
1.85 mL/min
Sample:
As noted

Glycan Analysis

The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the Biozen Glycan excels with increased polar retention and selectivity.

Intact Mass of Trastuzumab Biosimilar using a Biozen Intact XB-C8 and SCIEX® X500B

Application ID 25037
Conditions for all columns:

Column:
Biozen 2.6 µm Glycan
Dimensions
150 x 2.1 mm
Part No.:
Mobile Phase:
A: 100 mM Ammonium Formate,  pH 4.5
B: Acetonitrile
Gradient:
Time (min)
0
10
24
38.5
38.6
40.6
40.7
48
 
% B
78
74.5
72
55.9
40
40
78
78
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
FLD ex/em 285/345 nm
Sample:
As noted

Oligonucleotide Solid Phase Extraction (SPE)

N-Gycan Clean-Up HILIC Solid Phase Extraction (SPE)

High recovery of labeled released N-glycans in a microelution format allowing for streamlined processing and clean-up of small sample volumes.