SP
165
2421
biozenspe-sample-preparation-products
biozen-magbeads
Biozen MagBeads
Immunocapture of mAbs Using Magnetic Beads
Biozen MagBeads are used for the purification, clean up, and isolation of proteins and peptide molecules using a paramagnetic affinity bead with a streptavidin coated surface. Magnetic beads offer a rapid solution compared to traditional sample preparation options by maximizing binding capacity with a uniform particle for accurate and reliable results, in less time.
smooth-scroll-vertical-tabs
Order
Non-saturated, accurate binding surface orientation
Blocked with hydrophilic copolymer and coupled with biotinylated BSA which:
- Properly orients capture antibody
- Prevents surface crowding and ligand inactivation through non-specific binding
- Enhances dispersion of particles
- Improves binding efficiency
MagBead Protocol
MagBead Activation
Immunocapture
Rituximab Signature Peptide - ASGYTFTSYNMHWVK
Comparison of Dynabeads M-280 vs. Biozen MagBeads
Column:
Biozen 3 µm Peptide PS-C18
Dimension:
50 x 2.1 mm
Flow Rate:
0.3 mL/min
Temperature:
40 °C
Detection:
SCIEX X500B Q-TOF
Mobile Phase:
A: 0.1 % Formic acid in Water, B: 0.1 % Formic acid in Acetonitrile
Gradient:
3-50 % B in 4.5 minutes
Part No.:
Sample:
Rituximab 1.5 µg/mL (ASGYTFTSYNMHWVK)
Biozen MagBeads Binding Activity Leads to
Accurate and Sensitive Results
Rituximab
- Reduction in non-specific binding
- Excellent reproducibility lot-to-lot
Troponin
- Increased assay precision
- Improvements in response
Immunocapture Bead
Correlation Coefficient
Thermo® Dynabeads M-280
0.9176
Biozen MagBeads, Lot 1
0.9914
Biozen MagBeads, Lot 2
0.9941
Comparative separations may not be representative of all applications.
Concentration
(ng/mL)
(ng/mL)
MyOne™ Streptavidin T1 % CV Cal Dose
Biozen MagBeads
% CV Cal Dose
% CV Cal Dose
0.3
1
1.8
1.2
1.8
0.7
5
3.6
0.4
25
3.2
1
50
4.6
1.7
Documents
View All Documents
Webinars
View All Webinars