Biozen N-Glycan Clean-Up
Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)
SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.
- Small volume format allows for more concentrated samples
- Minimal elution solvent
- Excellent retention and recovery of labeled, released N-glycans
- Faster clean-up methods to save on sample prep time
Order
Overview
Protocol
N-Glycan Clean-up
Reliable recovery and faster results
STEP 1
Start with Intact Glycoprotein
STEP 2
Deglycosylation Released N-Glycans by PNGase F in buffer solution
STEP 3
Labeling Glycans 2-AA or 2-AB for accurate and sensitive glycan detection
STEP 4
Clean-Up Removal of labeling reagent, salts, or surfactants
STEP 5
Analysis
Labeled Glycans from AGP
