Clarity
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claritylc-hplc-column
Clarity HPLC/PREP Columns
Each product in Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.
Features of Clarity HPLC Columns for Oligonucleotides
- Easily separate N-1 failure sequences from target oligo with > 90% purities
- Trityl-off purification of DNA, RNA, Thioates, and modified/labeled oligonucleotides
- Preparative dimensions and particle sizes for loads > 5μmol
- Purify oligos up to 60 mer in length
- Excellent column for reverse phase HPLC quality control (QC) testing
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Select your ideal Clarity HPLC Columns phase based on type of separation or workflow
PRODUCT FINDER
Overview
LC-MS Analysis
Clarity Oligo-XT
- First choice for analytical to preparative scale chromatography
- pH stable 1-12
- 100% aqueous mobile phase stable
- Core-shell particle technology provides improved speed, resolution, and sensitivity
- Easily scale analytical LC-MS methods to preparative scale for isolation/purification
- 1.7µm particles offer increased performance over existing sub-2µm methods
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Clarity Oligo-MS
- Select if alternative selectivity is needed for analytical scale chromatography
- pH stable from 1.5-8.5
- Core-shell particle technology provides improved speed, resolution, and sensitivity
- Easily transfer quantitative LC-MS methods to any system with 2.6µm particles
- 1.7µm particles offer increased performance over existing sub-2µm methods
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Clarity Oligo-RP
- Select if alternative selectivity is needed for analytical to preparative scale chromatography
- pH stable from 1.0-12
- Silica-organic grafting to create composite particle for improved robustness
- Easily scale analytical LC-MS methods to preparative scale for isolation/purification
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Material Characteristics
Clarity Products Porous
Particle Support
Bonded Phase
Particle Shape/Size (μm)
Pore Size(Å)
Surface Area (m2/g)
TWIN (silica, organic composite) C18 3, 5, 10 110 375
C18
3, 5, 10
110
375
Clarity BioSolutions Product Selection
Purification
Clarity QSP™
Clarity Oligo-RP™ LC Columns
Clarity Oligo-XT LC Columns
Clarity RP-Desalting™
Primary Use
High-throughput, trityl-on RPC purification
RP-HPLC purification of failure
sequences from target sequences
Quick removal or salt & excess reagent
Purities
>90%
>90%
~70%
Recoveries
~90%
~70%
~70%
Synthesis Scale Load
Up to 50 µmol
Up to 50 µmol
Up to 1 µmol
Oligo Types
DNA, RNA/RNAi, Thioates, Dye-labled, Modified
Characterization / Analysis
Clarity Oligo-RP™ LC Columns
Clarity Oligo-MS™ LC Columns
Clarity Oligo-XT LC Columns
Clarity OTX™ LC Columns
Primary Use
RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length
≤ 60 mer
≤ 60 mer
≤ 40 mer
Recommended Mobile Phase
TEA / HFIP
TEA/HFIP/MeOH
N/A
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