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Kinetex Core-Shell Technology Columns
Kinetex core-shell technology offers Completely Scalable HPLC Column Solutions from UHPLC to HPLC to PREP LC. Kinetex core-shell column selectivities include reversed phase (C18, phenyl-hexyl, biphenyl, pentafluorophenyl, C8), and HILIC.
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Kinetex Core-Shell Technology Columns

The Core-Shell Advantage

Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running HPLC or UHPLC methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles. The combination of a consistent, solid high density core along with proprietary column packing technologies ensures optimum bed structure and high column performance.

  • Performance gains on ANY LC system
  • System-to-system and lab-to-lab method portability
  • Improve the productivity of older, established methods 


Kinetex® Core-Shell Technology Columns
Kinetex Core-Shell Technology Columns

Kinetex Core-Shell Performance on Any LC System

Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.

Optimized for Ultra-High Performance

Core-Shell Performance Gains

Kinetex Core Shell Technology finely tuned and unique core shell manufacturing process delivers dramatic improvements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consumption, and decrease costs Whether you are running HPLC or UHPLC methods, the Kinetex core shell family can deliver shockingly improved performance over the current column you are using.

Illustration of Eddy Diffusion Effects

Kinetex Polar C18

Fully Porous

High Efficiency‚ High Density Particle

Kinetex particles are built with a solid high density core that promotes the particles to settle into an optimal bed structure. This reduces the band broadening effects of Eddy Diffusion since the interstitial space between the particles is virtually homogeneous and results in ultra-high column efficiency and excellent reproducibility.

Achieve Sub-2 µm Performance within HPLC Backpressure Limitations

With the efficiency of a sub-2 µm column and typical operating backpressure less than 400 bar†‚ you can achieve the promise of ultra-high performance on any LC system.

† Kinetex 2.6 μm columns‚ 2.1 mm ID‚ are pressure rated to 1000 bar use on both HPLC and UHPLC instrumentation.
Conditions for both columns:

Columns:
Kinetex 2.6 µm C18
Traditional 1.7 µm C18
Dimension:
50 x 2.1 mm
Mobile Phase:
Acetonitrile / Water (50:50)
Flow Rate:
1.0 mL/min
Temperature:
25 °C
Detection:
UV @ 254 nm
Instrument:
Waters® ACQUITY® UPLC®
Sample:
0.5 µL test mixture
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene

Superior Core-Shell Quality

Uniform Particle Size Distribution

Kinetex particles are nearly monodispersed. This extremely narrow particle size distribution results in increased column efficiency and excellent reproducibility.

Batch-to-Batch Reproducibility Overlay

Columns:
Kinetex 2.6 µm C18
Dimension:
50 x 4.6 mm
Part No.:
00B-4462-E0
Mobile Phase:
Water / Acetonitrile (65:35)
Flow Rate:
1.0 mL/min
Temperature:
25 °C
Detection:
UV @ 254 nm
Sample:
1. Uracil
2. Hydrocortisone
3. Cortisone
4. Cortisone acetate
5. 17-Hydroxyprogesterone

Scalability: UHPLC/HPLC/PREP LC

With Kinetex 5µm, 2.6µm, 1.7µm, and 1.3µm Core-Shell Technology, you are no longer restricted from developing high performance LC methods on any system and transferring them anywhere. These four scalable Kinetex particle sizes offer you the ability to develop and transfer your method effortlessly from system to system.

Gingerols

Conditions are the same except as noted:

Columns:
Kinetex 5 μm C18
Dimension:
250 x 4.6 mm
Part No.:
00G-4601-E0

Portability: Kinetex 5 µm C18 on Shimadzu® LC-20A

Columns:
Kinetex 5 μm C18
Dimension:
250 x 4.6 mm
Part No.:
00G-4601-E0

Portability: Kinetex 2.6 µm C18 on Agilent® 1100

Columns:
Kinetex 2.6 μm C18
Dimension:
250 x 4.6 mm
Part No.:
00F-4462-E0

Portability: Kinetex 1.7 µm C18 on Agilent 1290

Columns:
Kinetex 1.7 μm C18
Dimension:
50 x 3.0 mm
Part No.:
00B-4475-Y0
Mobile Phase:
680:320:2

Expand Your Core-Shell Selectivity Toolbox

Kinetex core-shell particle LC columns provide unparalleled performance gains on ANY LC system! Plus, you have your pick of 11 amazing stationary phases and 5 versatile particle sizes!
UHPLC
UHPLC
UHPLC/HPLC
HPLC
HPLC/PREP

Hydrophobic Compounds

Kinetex C18 is a well-rounded, all-purpose core-shell column that produces increased efficiencies over traditional fully porous columns. Yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so labs can get the most out of every HPLC or UHPLC analysis.

Core-Shell vs Fully Porous

Comparative separations may not be representative of all applications.
Conditions for all columns:

Column:
Kinetex 2.6 µm C18
HYPERSIL GOLD 3 µm C18
Waters XBridge 3 µm C18
Dimensions:
150 x 4.6 mm
Mobile Phase:
A: Water with 0.1 % of Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
Gradient:
Time (min)
0
1
7
7.01
11
 
% B
15
15
35
15
15
Flow Rate:
1.2 mL/min
Injection Volume:
5 µL
Tempperature:
30 °C
Detection:
UV @ 230 nm

Polar Acidic Compounds 

The Kinetex 2.6 μm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 μm and 3 μm methods to shorten run runtime, increase sensitivity, and even provide greater resolution with much higher efficiency levels. The Kinetex Polar C18 is an excellent all-purpose, 100 % aqueous stable phase for use with multi-compound mixes that contain polar and non-polar compounds, or even single class methods that have closely related compounds, impurities, or metabolites.

Water Soluble Vitamins

Comparative separations may not be representative of all applications.

Column:
Kinetex 2.6 µm Polar C18
AQUA 3 µm C18
Dimensions:
150 x 4.6 mm
Mobile Phase:
A: 20 mM Potassium Phosphate (pH 1.5)
B: Methanol
Gradient:
Time (min)
0
1
7
7.01
14
 
% B
0
30
30
0
0
Flow Rate:
1.2 mL/min
Injection Volume:
5 µL
Tempperature:
22 °C
Detection:
UV @ 210 nm
Sample:
1. Thiamine
2. Nicotinamide
3. Pyridoxal
4. Pyridoxine
5. Pantothenic Acid

Polar Basic Compounds

Improved Impurity Loading Profiling for Bases

While traditional alkyl C18 phases are prone to peak tailing for basic compounds due to of secondary interactions occurring at the silica surface, the surface of the Kinetex PS C18 was designed with positive charges that serve to repel basic species and deliver consistently sharper peak shape for basic compounds.

Conditions for both columns:
Column:
Kinetex 2.6 µm PS C18
Core-Shell 2.6 µm C18
Dimensions:
50 x 4.6 mm
Mobile Phase:
A: Methanol with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
Gradient:
Time (min)
0
2
3
3.1
5
 
% B
25
35
95
25
25
Flow Rate:
1.85 mL/min
Temperture:
Ambient
Detection:
UV @ 254 nm
Sample:
1. Imipramine
2. Amitriptyline

Alkaline Mobile Phases Conditions

Kinetex EVO C18 uses a patented organo-silica grafting process which incorporates uniform stabilizing ethane crosslinking to provide resistance to high pH attack while maintaining the mechanical strength of the core-shell particle. Providing both increased peak sensitivity and decreased overall analysis time.

Comparative separations may not be representative of all applications.
Conditions for all columns:

Column:
Kinetex 5 µm EVO C18
XBridge 5 µm C18
Dimensions:
150 x 4.6 mm
Mobile Phase:
20 mM Sodium Phosphate/ Methanol/Acetonitrile (30:35:35)
Flow Rate:
1.25 mL/min
Temperature:
30 °C
Detection:
UV @ 254 nm
Sample:
1. Protriptyline
2. Nortriptyline
3. Imipramine
4. Amitriptyline
5. Clomipramine

Closely Related Compounds

Separation of Closely Related Compounds

Think high performance‚ enhanced retention‚ and the ability to go where a traditional C18 can’t. The Kinetex Biphenyl offers the high performance benefits of a core-shell particle with a unique stationary phase capable of becoming the go-to selectivity for reversed  phase method development. Use Kinetex Biphenyl columns to get enhanced retention‚ higher sensitivity‚ and overall better results; especially for aromatic compounds.

With the astonishing combination of core-shell performance and 5 interaction mechanisms: Hydrophobic; Aromatic; Electrostatic; Steric/Polar; and Hydrogen Bonding, Kinetex F5 columns will effortlessly drive your orthogonal HPLC/UHPLC development!

Kinetex Biphenyl 21992_21993
Conditions for all columns:

Column:
Kinetex 2.6 µm Biphenyl
HALO 2.7 µm C18
Dimensions:
50 x 2.1 mm
Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Methanol with 0.1 % Formic Acid
Gradient:
Time (min)
0
0.5
5
4.5
4.51
5.5
5.51
7
 
% B
0
0.5
2
4.5
4.51
5.5
5.51
7
Flow Rate:
1.85 mL/min
Temperature:
30 °C
Detection:
UV @ 254 nm
Sample:
1. Imipramine
2. Amitriptyline

Performance and Selectivity

Comparative separations may not be representative of all applications.
Conditions for all columns:

Column:
Kinetex 2.6 µm F5
HALO 2.7 µm PFP
XSelect HSS 2.5 µm PFP</div>
Dimensions:
100 x 4.6 mm
Mobile Phase:
Water with 0.1 % Formic Acid/ Methanol with 0.1 % Formic Acid (15:85)
Flow Rate:
0.75 mL/min
Temperature:
Ambient
Detection:
MS/MS (SCIEXâ„¢ API 4000â„¢)
Sample:
1. 25-OH Vitamin D3
2. 25-OH Vitamin D2
3. 3-epi-25-OH Vitamin D3

Material Characteristics

Kinetex Phases
Shipping Solvent†
Particle
Sizes
(µm)
Pore
Size
(Ã…)
Surface
Area
(m2/g)
Carbon
Load
(%)
pH Stability
Reversed
Phase
Normal
Phase
HILIC
100%
Aqueous
Stable
Polar C18
Acetonitrile / Water (50:50)
2.6
100
200
9
1.5-8.5*


PS C18
Acetonitrile / Water (50:50)
2.6
100
200
9
1.5-8.5*


C18
Acetonitrile / Water (50:50)
1.3, 1.7, 2.6, 5
100
200
12
1.5-8.5*



EVO C18
Acetonitrile / Water (45:55)
1.7, 2.6, 5
100
200
11
1-12


XB-C18
Acetonitrile / Water (50:50)
1.7, 2.6, 3.5, 5
100
200
10
1.5-8.5*



C8
Acetonitrile / Water (45:55)
1.7, 2.6, 5
100
200
8
1.5-8.5*



Biphenyl
Acetonitrile/Water w/
0.1 % Formic Acid (50:50)
1.7, 2.6, 5
100
200
11
1.5-8.5*


Phenyl-Hexyl
Acetonitrile / Water (45:55)
1.7, 2.6, 5
100
200
11
1.5-8.5*



F5
Acetonitrile / Water (40:60)
1.7, 2.6, 5
100
200
9
1.5-8.5*

HILIC
Acetonitrile /
100 mM Ammonium Formate (93:7)
1.7, 2.6, 5
100
200
0
2.0-7.5

PAH
Acetonitrile / Water (65:35)
3.5
—
—
12
1.5-8.5*




† Shipping conditions may vary slightly in terms of organic to aqueous ratio, depending on column dimensions. * pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
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