Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running HPLC or UHPLC methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles. The combination of a consistent, solid high density core along with proprietary column packing technologies ensures optimum bed structure and high column performance.
Kinetex Core Shell Technology finely tuned and unique core shell manufacturing process delivers dramatic improvements in efficiency over conventional fully porous media which can be leveraged to increase resolution, greatly improve productivity, reduce solvent consumption, and decrease costs Whether you are running HPLC or UHPLC methods, the Kinetex core shell family can deliver shockingly improved performance over the current column you are using.
Kinetex particles are built with a solid high density core that promotes the particles to settle into an optimal bed structure. This reduces the band broadening effects of Eddy Diffusion since the interstitial space between the particles is virtually homogeneous and results in ultra-high column efficiency and excellent reproducibility.
Columns:
Kinetex 2.6 µm C18
Traditional 1.7 µm C18 |
Dimension:
50 x 2.1 mm
|
Mobile Phase:
Acetonitrile / Water (50:50)
|
Flow Rate:
1.0 mL/min
|
Temperature:
25 °C
|
Detection:
UV @ 254 nm
|
Instrument:
Waters® ACQUITY® UPLC®
|
Sample:
0.5 µL test mixture
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
|
Kinetex particles are nearly monodispersed. This extremely narrow particle size distribution results in increased column efficiency and excellent reproducibility.
Columns:
Kinetex 2.6 µm C18
|
Dimension:
50 x 4.6 mm
|
Part No.:
00B-4462-E0
|
Mobile Phase:
Water / Acetonitrile (65:35)
|
Flow Rate:
1.0 mL/min
|
Temperature:
25 °C
|
Detection:
UV @ 254 nm
|
Sample:
1. Uracil
2. Hydrocortisone
3. Cortisone
4. Cortisone acetate
5. 17-Hydroxyprogesterone
|
Columns:
Kinetex 5 μm C18
|
Dimension:
250 x 4.6 mm
|
Part No.:
00G-4601-E0
|
Columns:
Kinetex 5 μm C18
|
Dimension:
250 x 4.6 mm
|
Part No.:
00G-4601-E0
|
Columns:
Kinetex 2.6 μm C18
|
Dimension:
250 x 4.6 mm
|
Part No.:
00F-4462-E0
|
Columns:
Kinetex 1.7 μm C18
|
Dimension:
50 x 3.0 mm
|
Part No.:
00B-4475-Y0
|
Mobile Phase:
680:320:2
|
Column:
Kinetex 2.6 µm C18
HYPERSIL GOLD 3 µm C18 Waters XBridge 3 µm C18 |
|
Dimensions:
150 x 4.6 mm
|
|
Mobile Phase:
A: Water with 0.1 % of Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
|
|
Gradient:
Time (min)
0
1
7
7.01
11
|
% B
15
15
35
15
15
|
Flow Rate:
1.2 mL/min
|
|
Injection Volume:
5 µL
|
|
Tempperature:
30 °C
|
|
Detection:
UV @ 230 nm
|
Column:
Kinetex 2.6 µm Polar C18
AQUA 3 µm C18 |
|
Dimensions:
150 x 4.6 mm
|
|
Mobile Phase:
A: 20 mM Potassium
Phosphate (pH 1.5)
B: Methanol |
|
Gradient:
Time (min)
0
1
7
7.01
14
|
% B
0
30
30 0
0
|
Flow Rate:
1.2 mL/min
|
|
Injection Volume:
5 µL
|
|
Tempperature:
22 °C
|
|
Detection:
UV @ 210 nm
|
|
Sample:
1. Thiamine
2. Nicotinamide 3. Pyridoxal 4. Pyridoxine 5. Pantothenic Acid |
While traditional alkyl C18 phases are prone to peak tailing for basic compounds due to of secondary interactions occurring at the silica surface, the surface of the Kinetex PS C18 was designed with positive charges that serve to repel basic species and deliver consistently sharper peak shape for basic compounds.
Column:
Kinetex 2.6 µm PS C18
Core-Shell 2.6 µm C18
|
|
Dimensions:
50 x 4.6 mm
|
|
Mobile Phase:
A: Methanol with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
|
|
Gradient:
Time (min)
0
2
3
3.1
5
|
% B
25
35
95
25
25
|
Flow Rate:
1.85 mL/min
|
|
Temperture:
Ambient
|
|
Detection:
UV @ 254 nm
|
|
Sample:
1. Imipramine
2. Amitriptyline
|
Column:
|
|
Dimensions:
150 x 4.6 mm
|
|
Mobile Phase:
20 mM Sodium Phosphate/
Methanol/Acetonitrile
(30:35:35)
|
|
Flow Rate:
1.25 mL/min
|
|
Temperature:
30 °C
|
|
Detection:
UV @ 254 nm
|
|
Sample:
1. Protriptyline
2. Nortriptyline 3. Imipramine 4. Amitriptyline 5. Clomipramine |
Think high performance‚ enhanced retention‚ and the ability to go where a traditional C18 can’t. The Kinetex Biphenyl offers the high performance benefits of a core-shell particle with a unique stationary phase capable of becoming the go-to selectivity for reversed phase method development. Use Kinetex Biphenyl columns to get enhanced retention‚ higher sensitivity‚ and overall better results; especially for aromatic compounds.
With the astonishing combination of core-shell performance and 5 interaction mechanisms: Hydrophobic; Aromatic; Electrostatic; Steric/Polar; and Hydrogen Bonding, Kinetex F5 columns will effortlessly drive your orthogonal HPLC/UHPLC development!
Column:
Kinetex 2.6 µm Biphenyl
HALO 2.7 µm C18 |
|
Dimensions:
50 x 2.1 mm
|
|
Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Methanol with 0.1 % Formic Acid
|
|
Gradient:
Time (min)
0
0.5
5
4.5 4.51 5.5 5.51 7
|
% B
0
0.5
2
4.5 4.51 5.5 5.51 7
|
Flow Rate:
1.85 mL/min
|
|
Temperature:
30 °C
|
|
Detection:
UV @ 254 nm
|
|
Sample:
1. Imipramine
2. Amitriptyline
|
Column:
|
|
Dimensions:
100 x 4.6 mm
|
|
Mobile Phase:
Water with 0.1 % Formic Acid/ Methanol with 0.1 % Formic Acid (15:85)
|
|
Flow Rate:
0.75 mL/min
|
|
Temperature:
Ambient
|
|
Detection:
MS/MS (SCIEXâ„¢ API 4000â„¢)
|
|
Sample:
1. 25-OH Vitamin D3
2. 25-OH Vitamin D2 3. 3-epi-25-OH Vitamin D3 |
Kinetex Phases
|
Shipping Solventâ€
|
Particle
Sizes (µm) |
Pore
Size (Ã…) |
Surface
Area (m2/g) |
Carbon
Load (%) |
pH Stability
|
Reversed
Phase |
Normal
Phase |
HILIC
|
100%
Aqueous Stable |
---|---|---|---|---|---|---|---|---|---|---|
Polar C18
|
Acetonitrile / Water (50:50)
|
2.6
|
100
|
200
|
9
|
1.5-8.5*
|
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||
PS C18
|
Acetonitrile / Water (50:50)
|
2.6
|
100
|
200
|
9
|
1.5-8.5*
|
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||
C18
|
Acetonitrile / Water (50:50)
|
1.3, 1.7, 2.6, 5
|
100
|
200
|
12
|
1.5-8.5*
|
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|||
EVO C18
|
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
11
|
1-12
|
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||
XB-C18
|
Acetonitrile / Water (50:50)
|
1.7, 2.6, 3.5, 5
|
100
|
200
|
10
|
1.5-8.5*
|
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|||
C8
|
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
8
|
1.5-8.5*
|
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|||
Biphenyl
|
Acetonitrile/Water w/
0.1 % Formic Acid (50:50) |
1.7, 2.6, 5
|
100
|
200
|
11
|
1.5-8.5*
|
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||
Phenyl-Hexyl
|
Acetonitrile / Water (45:55)
|
1.7, 2.6, 5
|
100
|
200
|
11
|
1.5-8.5*
|
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|||
F5
|
Acetonitrile / Water (40:60)
|
1.7, 2.6, 5
|
100
|
200
|
9
|
1.5-8.5*
|
![]() |
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|
HILIC
|
Acetonitrile /
100 mM Ammonium Formate (93:7) |
1.7, 2.6, 5
|
100
|
200
|
0
|
2.0-7.5
|
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||
PAH
|
Acetonitrile / Water (65:35)
|
3.5
|
—
|
—
|
12
|
1.5-8.5*
|
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