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Luna HILIC HPLC Columns

The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.

  • Reproducible‚ robust HILIC separations
  • Made for retention of polar compounds
  • Increase mass spectrometry sensitivity
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HPLC
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Overview

Long Column Lifetimes and Excellent Performance

Ultra-pure, metal-free silica (99.99 (= 99.99 %) purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

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Agilent Technologies® ZORBAX®
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Waters® Symmetry®
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Phenomenex Luna
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Increase MS Sensitivity and Retention for Polar Compounds

Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode where the partitioning of polar solutes from the high concentration‚ water-miscible‚ organic mobile phase into the hydrophilic surface environment creates separations. Polar solutes exhibit increased retention and elute in the order of increasing hydrophilicity.

Water Soluble Vitamins

Conditions:

Column:
Luna 5 μm HILIC
Dimension:
150 x 4.6 mm
Flow Rate:
1.0  mL/min
Temperature:
Ambient
Detection:
UV @ 260 nm
Mobile Phase:
Acetonitrile/100 mM, Ammonium Formate, pH 3.2 (90:10)

Sample:

1. PABA
2. Nicotinamide
3. Riboflavin
4. Nicotinic Acid
5. Pyridocine
6. Thiamine

25942, 25752, 25436

Applications

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