Luna PFP(2) HPLC Columns
The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds, while the rigid bonded phase is a good steric selector.
- Extremely discerning for halogenated‚ aromatic and conjugated compounds
- Provides orthogonal selectivity even using traditional reversed phase mobile phase systems
- PFP Ultra pure, metal-free silica (99.99 % purity) (USP: L43)
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Overview
Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.
Superior Particle Smoothness
4 Mechanisms of Interaction
Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.
- Hydrogen Bonding
- Dipole-Dipole Interactions
- Aromatic and π-π Interactions
- Hydrophobic
A typical alkyl phase (C18‚ C8) achieves selectivity through only 1 mechanism of interaction.
Isomeric Compounds
Positional Isomers of Methylacetophenone
Positional changes on an analyte of interest may effect the compound’s dipole moment. This change can be readily seen by the way the highly electronegative fluorine (F) atoms and other retention mechanisms of the Luna PFP(2) are able to separate positional isomers.
The high efficiencies and bonded phase surface coverage provide for sharp peaks The C 8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.
Luna 3 µm PFP(2)
Sample:
- o-Methylacetophenone
- m-Methylacetophenone
- p-Methylacetophenone
Aromatic Compounds
Catechins
Aromatic compounds show unique retention characteristics on Luna PFP(2) compared to traditional reversed phase columns. The presence of the aromatic benzene ring in Luna PFP(2) increases the relative attraction between the stationary phase and aromatic analytes, leading to increased retention for these types of compounds.
Luna 3 µm PFP(2)
Conditions same for both columns:
A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Sample:
- Gallic acid
- Epigallo catechin
- Catechin
- Epicatechin
- Epigallocatechin gallate
- Gallocatechin gallate
- Epicatechin gallate
- Catechin gallate