Luna Omega Polar C18 HPLC Columns

Reversed Phase Polar Retention Without the Headache

Luna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes. The all purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.

  • Compatible with All Analytical HPLC/UHPLC Systems*
  • Fully Scalable Particle Size UHPLC HPLC Preparative
  • 100 % Aqueous Stable C18 Selectivity

Scalable Particle Sizes

Hydrophobic Selectivity

*Luna Omega analytical columns are compatible with all LC system with standard LC column end fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.

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Overview

Not Just Another C18…Built Different

Luna Omega Polar C18 is a uniquely modified C18 based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize HPLC for mixtures of polar and non polar compounds.

Another C18 Built Different

Stable Retention & 100% Aqueous Stable

Traditional C18 phases are known to collapse under 100 % aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna Omega Polar C18 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C18 in 100% aqueous buffer conditions for over 2 weeks.

Stable Retention & 100% Aqueous Stable

Conditions for all columns:

Columns:
Luna Omega 1.6 µm Polar C18
Dimension:
50 x 2.1 mm
Flow Rate:
0.4 mL/min
Temperature:
22 °C
Detection:
UV @ 254 nm
Mobile Phase:
10 mM Ammonium Formate with 0.1 % Formic Acid
Part No.:
00B-4748-AN

Samples:

  1. Adenine
  2. Guanosine-5-Monophosphate
  3. Thymine
Duration of Exposure
Day 1 , Day 25

Increased Reversed Phase Polar Retention

With its polar modified surface, the Luna Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.

Pharmaceutical Compound Mixture

Pharmaceutical Compound Mixture

Columns:
Luna Omega 1.6 µm Polar C18 , Kinetex 1.7 µm C18
Dimension:
50 x 2.1 mm
Flow Rate:
0.4 mL/min
Temperature:
22 °C
Detection:
MS/MS (SCIEX API 4000™)
Mobile Phase:
A: 10 mM Ammonium Formate with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
Injection Volume:
1 µL
Gradient : Time (min)
% B
0
0
3
90

Samples:

  1. 3-Hydroxyphenol
  2. Phenol
  3. 4-Nitrophenol
  4. 4-Methylphenol
  5. 2-Methylphenol
  6. 2,4-Dimethylphenol
  7. 1-Naphthol

Dipeptides in 100 % Aqueous Conditions

Dipeptides Aqueous Conditions

Columns:
Luna Omega 1.6 µm Polar C18 , Competitor 1.7 µm C18
Dimension:
50 x 2.1 mm
Flow Rate:
0.6 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm (ambient)
Mobile Phase:
A: Water with 0.1 % TFA
B: Acetonitrile with 0.1 % TFA
Part No.:
00B-4748-AN
Injection Volume:
1 µL
Gradient : Time (min)
% B
0
0
3
75

Samples:

  1. Arg-Glu
  2. Gly-Tyr
  3. Trp-Gly
  4. Gly-Trp
  5. Pro-Trp
25942, 25752, 25436

Applications

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