Rezex RAM-Carbohydrate Ag+ (8%) Ion Exclusion HPLC Columns
- 8 % cross-linked silver ionic form
- Unique selectivity complementary to other Rezex column types
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Overview
Select Applications on Rezex HPLC Columns
Saccharides
Column:
Rezex RAM-Carbohydrate
Dimension:
300 x 7.8 mm
Part No.:
00H-0131-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
ELSD
Sample:
1. Melezitose
2. Mannose
3. Maltose
4. Fructose
5. Glucose
2. Mannose
3. Maltose
4. Fructose
5. Glucose
Carbohydrates
Column:
Rezex RAM-Carbohydrate
Dimension:
300 x 7.8 mm
Part No.:
00H-0131-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Injection Volume:
5 µL
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Sucrose
2. Glucose
3. Arabinose
4. Ribose
2. Glucose
3. Arabinose
4. Ribose
Applications (partial list)
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Compound
App ID No.
Arabinose
14974
Fructose
14966
Glucose
14966, 14974
Maltose
14966
Mannose
14966
Compound
App ID No.
Melezitose
14966
Ribose
14974
Ribitol
14966
Sucrose
14974
Specifications and Operating Recommendations
RAM Carbohydrate
Part No.
00H-0131-K0
Ionic Form
Silver
Standard Dimensions
300 x 7.8 mm
Matrix
Sulfonated Styrene Divinyl Benzene
Cross Linking
8 %
Particle Size (μm)
8
Min. Efficiency (p/m) based on last peak
35,000
Typical Pressure (psi @ Testing Flow Rate)
285
Max. Pressure (psi @ Max Flow Rate)
1,000
Max. Flow Rate (mL/min)
1.0
Max. Temperature (°C)
85
Typical Mobile Phase
Water
pH Range
Neutral
Guard Column Part
03B-0131-K0
Cleaning, Regeneration and Storage
Organic Modifiers (Max)
5 % Methanol, IPA, EtOH
Inorganic Modifiers
2 % Silver Nitrate
Avoid
Acids, Bases, Non-Silver Salts or
Metal Ions, > 30 % Acetonitrile
Metal Ions, > 30 % Acetonitrile
Cleaning Solvent
100 % Water
Flow Rate(mL/min)
0.4
Temperature (°C)
85
Duration (hrs)
12
Regeneration Solvent
0.1 M AgNO3
Flow Rate (mL/min)
0.2
Temperature (°C)
85
Duration (hrs)
4-16
Ship/Storage Solvent
Water
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