Rezex RCM-Monosaccharide Ca+2 (8%) Ion Exclusion HPLC Columns
- 8% cross-linked calcium
- For use with monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars
- Class separation of di-, tri-, and tetra- saccharides
- Easy regeneration with calcium nitrate solutions
HPLC
80
292
rezex-hplc-column
rezex-rcm-monosaccharide-ca
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Overview
Cross Reference Guide
Phase
Description
Applications
Bio-Rad® Aminex®
Supelco® Supelcogel™
Waters® Sugar-Pak™
Transgenomic® CARBOSep™
Sepax® Carbomi®
RCM-Monosaccharide (USP L19 packing)*
8 % cross-linked CALCIUM
– Monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars
– Class separation of di-, tri-, and tetra- saccharides
– Class separation of di-, tri-, and tetra- saccharides
HPX-87C
125-0095
125-0095
Supelcogel Ca
Sugar-Pak 1
CARBOSep CHO-820
Carbomix Ca
Sharper Peak Shape = Easy & Accurate Quantitation
Saccharides
Columns:
Rezex RCM-Monosaccharide,
Aminex HPX-87C
Aminex HPX-87C
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Mobile Phase:
Water
Sample:
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol
Comparative separations may not be representative of all applications.
Orange Juice
Columns:
Rezex RCM-Monosaccharide
Aminex HPX-87C
Aminex HPX-87C
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Mobile Phase:
Water
Sample:
1. Sucrose
2. Glucose
3. Fructose
Comparative separations may not be representative of all applications.
Higher Efficiency = Baseline Separation of Critical Sample Components
Sugars
Columns:
Rezex RCM-Monosaccharide
Bio-Rad® Aminex® HPX-87C
Bio-Rad® Aminex® HPX-87C
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Mobile Phase:
Water
Sample:
1. Sucrose
2. Glucose
3. Galactose
4. Fructose
5. Sorbitol
Comparative separations may not be representative of all applications.
Food and Beverage
Apple Juice
Columns:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0131-K0
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
RI
Mobile Phase:
Water
Sample:
1. Sucrose
2. Glucose
3. Fructose
Honey
Columns:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
RI
Mobile Phase:
Water
Sample:
1. Dp3
2. Dp2
3. Glucose
4. Fructose
Fermentation Broth
Columns:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Flow Rate:
0.5 mL/min
Temperature:
80 °C
Detection:
ELSD
Mobile Phase:
Water
Sample:
1. Sucrose
2. Glucose
3. Galactose
4. Fructose
Saccharides
Columns:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Flow Rate:
0.6 mL/min
Temperature:
85 °C
Detection:
RI
Mobile Phase:
Water
Sample:
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol
Other Applications
Food Softeners
Columns:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Flow Rate:
0.5 mL/min
Temperature:
60 °C
Detection:
RI
Mobile Phase:
Water
Sample:
1. Glycerol
2. Methoxypolyethylene Glycol
3. Triethylene Glycol
4. Sorbitol
5. Urea
Applications (partial list)
Call us for assistance in selecting the right column for your needs!
Compound
App ID No.
Fructose
5504, 5505, 14975, 14981,14977, 14987
Galactose
14977, 14987
Glucose
5504, 5505, 14975, 14977, 14979, 14981, 14987, 14983
Glycerol
5518
Maltose
5504, 14975
Mannose
5504, 14975
Melezitose
5504, 14975
Compound
App ID No.
Methoxypolyethylene Glycol
5518
Ribitol
5504, 14975
Sorbitol
5518, 14977
Sucrose
5505, 14981, 14977, 14987
Tartaric Acid
5504
Triethylene Glycol
5518
Urea
5518
Specifications and Operating Recommendations
Rezex RCM-Monosaccharide
Part No.
00H-0131-K0
Ionic Form
Calcium
Standard Dimensions
300 x 7.8 mm
Matrix
Sulfonated Styrene Divinyl Benzene
Cross Linking
8 %
Particle Size (μm)
8
Min. Efficiency (p/m) based on last peak
35,000
Typical Pressure (psi @ Testing Flow Rate)
260
Max. Pressure (psi @ Max Flow Rate)
1,000
Max. Flow Rate (mL/min)
1.0
Max. Temperature (°C)
85
Typical Mobile Phase
Water
pH Range
Neutral
Guard Column Part
03B-0131-K0
Cleaning, Regeneration and Storage
Organic Modifiers (Max)
5 % Methanol, IPA, EtOH
Inorganic Modifiers
5 % CaSO4, Ca(NO3)2, CaCl2
Avoid
Acids, Bases, Non-Silver Salts or
Metal Ions, > 30 % Acetonitrile
Metal Ions, > 30 % Acetonitrile
Cleaning Solvent
100 % Water
Flow Rate(mL/min)
0.4
Temperature (°C)
85
Duration (hrs)
12
Regeneration Solvent
0.1 M Ca(NO3)2
Flow Rate (mL/min)
0.2
Temperature (°C)
85
Duration (hrs)
4-16
Ship/Storage Solvent
Water
25942,25752,25436
Applications
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Documents
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