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Product Information
Phase Detail
Rezex RPM-Monosaccharide Pb<sup>+2</sup> Ion Exclusion HPLC Columns<br class="t-last-br" />
Rezex RPM-Monosaccharide Pb+2 Ion Exclusion HPLC Columns is packed with a 8% cross-linked lead ion exclusion media

Rezex RPM-Monosaccharide Pb+2 Ion Exclusion HPLC Columns

  • 8% cross-linked lead
  • Ideal for monosaccharide and sugar alcohol analysis
  • For separations of cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products
  • 100 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure

Particle Size
8
Recommended Use
Sugar alcohol analysis according to the USP procedure, monosaccharides and sugar alcohol analysis, cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products

Cross Reference Guide

Phase Description Applications Bio-Rad® Aminex® Supelco® Supelcogel™ Waters® Sugar-Pak™ Transgenomic® CARBOSep™ Sepax® Carbomi®
RPM-Monosaccharide (USP L34 packing) 8 % cross-linked LEAD – 100 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure
– Monosaccharides and sugar alcohol analysis
– Cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products
HPX-87P
125-0098
Supelcogel
Pb
N/A CARBOSep
COREGEL-87P
Carbomix
Pb

Retention Times for Some Carbohydrates and Sugar Alcohols

Counter Ion Analyte RPM Pb2+
Adonitol (Ribitol) 20.15
D-Altrose 15.82
D-(-)-Arabinose 16.47
D-(+)-Cellobiose 11.00
D-(+)-Digitoxose 15.32
Dulcitol 33.25
Meso-Erythritol 19.82
D-(-)-Fructose 17.71
L-(-)-Fucose 16.19
D-(+)-Galactose 14.94
Gentiobiose 10.53
D-(+)-Glucose 14.94
Inositol 18.87
Isomaltose 11.28
Lactose 11.89
Lactulose 13.95
D-Lyxose 16.66

Counter Ion Analyte RPM Pb2+
D-Maltose 11.59
Maltotriose 11.02
Maltulose 12.40
D-Mannitol 24.90
D-(+)-Mannose 16.39
Melibiose 11.97
D-(+)-Melezitose 9.94
D-(+)-Raffinose 10.28
L-(+)-Rhamnose 14.47
D-(-)-Ribose 33.48
Salicin 26.81
D-Sorbitol 35.97
Stachyose 9.72
Sucrose 11.00
Trehalose 11.01
Xylitol 32.38
D-(+)-Xylose 13.84

Sharper Peak Shape = Easy & Accurate Quantitation

Sugar Alcohols

Comparative separations may not be representative of all applications.

Column:
Rezex RPM-Monosaccharide
Bio-Rad® Aminex® HPX-87P
Dimension:
300 x 7.8 mm
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Glucose
2. Erythritol
3. Mannitol
4. Xylitol
5. Sorbitol

Lower Backpressure = Longer Column Lifetimes
and Faster Run Time Capability

Saccharides

Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.

Column:
Rezex RPM-Monosaccharide
Aminex HPX-87P
Dimension:
300 x 7.8 mm
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
ELSD
Sample:
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol

Select Applications on Rezex RPM-Monosaccharide

Wood Saccharides

Column:
Rezex RPM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0135-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
85 °C
Detection:
ELSD
Sample:
1. D-Cellobiose
2. α-D-glucose
3. D-(+)-Xylose
4. D-(+)-Galactose
5. D-(-)-Arabinose


Saccharides and Alcohols

Column:
Rezex RPM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0135-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
RI
Sample:
1. Stachyose
2. Maltose
3. Glucose
4. Xylose
5. Galactose
6. Fructose
7. meso-Erythritol
 
8. Mannitol
9. Salicin
10. Xylitol
11. Sorbitol


Oligosaccharides

Column:
Rezex RPM-Monosaccharide
Dimension:
100 x 7.8 mm
Part No.:
00D-0135-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD (Ambient)
Sample:
1. Mannitol
2. Sorbitol

Applications (partial list)

Call us for assistance in selecting the right column for your needs!

Compound App ID No.
D-Cellobiose 14967
D-(+)-Galactose 14967
D-(+)-Xylose 14967
D-(+)-Arabinose 14967
Erythritol 14968
Fructose 5508, 14985
Galactose 5508
Glucose 5508, 14985
Maltose 5508, 14985
Mannitol 5508, 14968

Compound App ID No.
Mannose 14985
Melezitose 14985
meso-Erythritol 5508
Ribitol 14985
Salacin 5508
Sorbitol 5508, 14968
Stachyose 5508
Xylitol 5508, 14968
Xylose 5508

Specifications and Operating Recommendations

Rezex RPM-Monosaccharide
Part No. 00H-0135-K0
Ionic Form Lead
Standard Dimensions 300 x 7.8 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8 %
Particle Size (μm) 8
Min. Efficiency (p/m) based on last peak 35,000
Typical Pressure (psi @ Testing Flow Rate) 190
Max. Pressure (psi @ Max Flow Rate) 1,000
Max. Flow Rate (mL/min) 1.0
Max. Temperature (°C) 85
Typical Mobile Phase Water
pH Range Neutral
Guard Column Part 03B-0135-K0
Cleaning, Regeneration and Storage
Inorganic Modifiers 5 % Lead Nitrile
Organic Modifiers (Max) 5% Methanol, IPA, EtOH
Avoid Acids, Bases, Non-Lead Salts or
Metal Ions, > 30 % Acetonitrile
Cleaning Solvent 100 % Water
Flow Rate(mL/min) 0.4
Temperature (°C) 85
Duration (hrs) 12
Regeneration Solvent 0.1 M Pb(NO3)2
Flow Rate (mL/min) 0.2
Duration (hrs) 4-16
Ship/Storage Solvent Water