Rezex RPM-Monosaccharide Pb+2 Ion Exclusion HPLC Columns

8% cross-linked lead
Ideal for monosaccharide and sugar alcohol analysis
For separations of cellobiose, glucose, xylose, and other cellulose products
100 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure

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HPLC

297

rezex-hplc-column

rezex-rpm-monosaccharide-pb

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Order

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Order

Overview

Cross Reference Guide

Phase

Description

Applications

Bio-Rad® Aminex®

Supelco® Supelcogel™

Waters® Sugar-Pak™

Transgenomic® CARBOSep™

Sepax® Carbomi®

RPM-Monosaccharide (USP L34 packing)

8 % cross-linked LEAD

– 100 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure
– Monosaccharides and sugar alcohol analysis
– Cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products

HPX-87P
125-0098

Supelcogel
Pb

N/A

CARBOSep
COREGEL-87P

Carbomix
Pb

Retention Times for Some Carbohydrates and Sugar Alcohols

Counter Ion Analyte

RPM Pb²⁺

Adonitol (Ribitol)

20.15

D-Altrose

15.82

D-(-)-Arabinose

16.47

D-(+)-Cellobiose

11.00

D-(+)-Digitoxose

15.32

Dulcitol

33.25

Meso-Erythritol

19.82

D-(-)-Fructose

17.71

L-(-)-Fucose

16.19

D-(+)-Galactose

14.94

Gentiobiose

10.53

D-(+)-Glucose

14.94

Inositol

18.87

Isomaltose

11.28

Lactose

11.89

Lactulose

13.95

D-Lyxose

16.66

Counter Ion Analyte

RPM Pb²⁺

D-Maltose

11.59

Maltotriose

11.02

Maltulose

12.40

D-Mannitol

24.90

D-(+)-Mannose

16.39

Melibiose

11.97

D-(+)-Melezitose

9.94

D-(+)-Raffinose

10.28

L-(+)-Rhamnose

14.47

D-(-)-Ribose

33.48

Salicin

26.81

D-Sorbitol

35.97

Stachyose

9.72

Sucrose

11.00

Trehalose

11.01

Xylitol

32.38

D-(+)-Xylose

13.84

Sharper Peak Shape = Easy & Accurate Quantitation

Sugar Alcohols

Comparative separations may not be representative of all applications.

Column:

Rezex RPM-Monosaccharide

Bio-Rad^® Aminex^® HPX-87P

Dimension:

300 x 7.8 mm

Flow Rate:

0.6 mL/min

Temperature:

80 °C

Detection:

ELSD

Mobile Phase:

Water

Sample:

1. Glucose
2. Erythritol
3. Mannitol
4. Xylitol
5. Sorbitol

Lower Backpressure = Longer Column Lifetimes and Faster Run Time Capability

Saccharides

Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.

Column:

Rezex RPM-Monosaccharide

Aminex HPX-87P

Dimension:

300 x 7.8 mm

Flow Rate:

0.6 mL/min

Temperature:

75 °C

Detection:

ELSD

Mobile Phase:

Water

Sample:

1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol

Select Applications on Rezex RPM-Monosaccharide

Wood Saccharides

Column:

Rezex RPM-Monosaccharide

Dimension:

300 x 7.8 mm

Flow Rate:

0.6 mL/min

Temperature:

85 °C

Detection:

ELSD

Mobile Phase:

Water

Part No.:

00H-0135-K0

Sample:

1. D-Cellobiose
2. α-D-glucose
3. D-(+)-Xylose
4. D-(+)-Galactose
5. D-(-)-Arabinose

Saccharides and Alcohols

Column:

Rezex RPM-Monosaccharide

Dimension:

300 x 7.8 mm

Flow Rate:

0.6 mL/min

Temperature

75 °C

Detection:

Mobile Phase:

Water

Part No.:

00H-0135-K0

Sample:

1. Stachyose
2. Maltose
3. Glucose
4. Xylose
5. Galactose
6. Fructose
7. meso-Erythritol
8. Mannitol
9. Salicin
10. Xylitol
11. Sorbitol

Oligosaccharides

Column:

Rezex RPM-Monosaccharide

Dimension:

100 x 7.8 mm

Flow Rate:

0.6 mL/min

Temperature:

80 °C

Detection:

ELSD (Ambient)

Mobile Phase:

Water

Part No.:

00D-0135-K0

Sample:

1. Mannitol
2. Sorbitol

Applications (partial list)

Call us for assistance in selecting the right column for your needs!

Compound

App ID No.

D-Cellobiose

D-(+)-Galactose

D-(+)-Xylose

D-(+)-Arabinose

Erythritol

Fructose

Galactose

Glucose

Maltose

Mannitol

Compound

App ID No.

Mannose

Melezitose

meso-Erythritol

Ribitol

Salacin

Sorbitol

Stachyose

Xylitol

Xylose

Specifications and Operating Recommendations

RAM Carbohydrate

Part No.

00H-0135-K0

Ionic Form

Lead

Standard Dimensions

300 x 7.8 mm

Matrix

Sulfonated Styrene Divinyl Benzene

Cross Linking

8 %

Particle Size (μm)

Min. Efficiency (p/m) based on last peak

35,000

Typical Pressure (psi @ Testing Flow Rate)

190

Max. Pressure (psi @ Max Flow Rate)

1,000

Max. Flow Rate (mL/min)

1.0

Max. Temperature (°C)

Typical Mobile Phase

Water

pH Range

Neutral

Guard Column Part

03B-0135-K0

Cleaning, Regeneration and Storage

Organic Modifiers (Max)

5 % Methanol, IPA, EtOH

Inorganic Modifiers

5 % Lead Nitrile

Avoid

Acids, Bases, Non-Lead Salts or
Metal Ions, > 30 % Acetonitrile

Cleaning Solvent

100 % Water

Flow Rate(mL/min)

0.4

Temperature (°C)

Duration (hrs)

Regeneration Solvent

0.1 M Pb(NO₃)₂

Flow Rate (mL/min)

0.2

Temperature (°C)

Duration (hrs)

4-16

Ship/Storage Solvent

Water

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