Strata NH2 SPE Products

Normal Phase Solid Phase Extraction (SPE)

This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.

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Overview

Available Formats

3
96-Well-Plate
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1, 3, and 6mL Tubes
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General Starting Method

Strata Tube Illustration

Strata NH2 – Weak Anion-Exchange

Condition
1 mL Methanol
Equilibrate
1 mL Water, pH 6-7
Load
Pretreated sample, pH 6-7
Wash 1
1 mL 25mM Ammonium Acetate Buffer, pH 6-7
Wash 2
1 mL Methanol, dry under vacuum for 2-5 min
Elute Any Acid
1 mL 5% NH4OH in Methanol
Elute Weak Acids
1 mL 5% Formic Acid in Methanol

Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

25942, 25752, 25436

Applications

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