SP
290
strata-solid-phase-extraction-products
Strata Solid Phase Extraction
Strata SPE products are manufactured to bring you optimal quality, reproducibility, and performance. With over 20 years of experience, Strata SPE products provide clean extracts and high recoveries for a multitude of target analytes across diverse sample matrices.
- Available retention mechanisms include reversed phase, ion exchange, normal phase and mixed mode
- 24 phases and counting
- Cleaner extracts and reproducible results
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Overview
Available Formats
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Silica-Based Sorbents Loading Capacities
Sample Matrix
Sorbent Mass
Blood, serum, plasma
50 mg sorbent per 250 µL
Urine
50 mg sorbent per 500 µL
Filtered tissue homogenates
100 mg sorbent per 100 mg tissue
Environmental Samples
Sorbent Mass
Water (particulate-free) drinking
500 mg/100 mL - 500 mL sample
Water (particulate-laden) rivers, runoff, etc.
1 g/100 mL - 500 mL sample
Soil extracts
1 g/100 g of soil extract
Food/Plant Material
1 g/ 100g sample
Sorbent Wash and Elution Volumes
The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.
Sorbent Mass
10 mg
50 mg
100 mg
150 mg
200 mg
500 mg
1 g
2 g
5 g
10 g
Practical Minimum Wash and
Elution Volume 4 bed volumes
Elution Volume 4 bed volumes
60 µL
300 µL
600 µL
900 µL
1.2 mL
3 mL
6 mL
12 mL
30 mL
60 mL
Recommended Wash and
Elution Volume 8 bed volumes
Elution Volume 8 bed volumes
120 µL
600 µL
1.2 mL
1.8 mL
2.4 mL
6 mL
12 mL
24 mL
60 mL
120 mL
General Starting Method Reversed Phase
Condition:
1 mL Methanol
Equilibrate:
1 mL DI Water
Load:
Pretreated sample
Wash:
1 mL 5% Methanol in DI Water, dry under vacuum for 2-5 min
Elute:
1 mL Methanol
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