Strata-XL-AW SPE Products

Large Particle Weak Anion Mixed-Mode Solid Phase Extraction (SPE)

A large particle, weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format makes this sorbent ideal for processing large volumes or viscous samples.

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SP
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2137
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Overview

3 Mechanism of Retention

4
Weak Anion-Exchange
false
π-π Bonding
false
Hydrophobic Interaction
false

Available Formats

4
96-Well-Plate
false
1, 3, and 6mL Tubes
false
Giga Tubes (12-150mL Tubes)
false

Starting Method

For Acids with pKa ≤ 5

Column
1mL Methanol
Equilibrate
1 mL Water, pH 6-7
Load
Diluted Sample pH 6-7
Wash 1
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
Wash 2
1 mL Methanol
Elute Acids
2x 500 µL 5 % NH4OH in Methanol
Elute Weak Acids
2x 500 µL 5 % Formic Acid in Methanol

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

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Applications

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