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The Analysis of 12 Cannabinoids in Whole Blood by LC/MS/MS

In addition to the psychoactive cannabinoid THC, there is a growing interest in researching the potential medicinal benefits of the plant’s other major cannabinoids, which are non-psychoactive. Cannabinoids are very hydrophobic compounds that elute late in reversed-phase chromatography. Since phospholipids also elute in this region, matrix effects and ion suppression can be a concern for the analysis of these compounds. This research presents an LC-MS/MS method for the analysis of 12 cannabinoids and 2 metabolites from whole blood. The objective is to present a simple sample preparation technique utilizing a phospholipid removal cartridge to facilitate the removal of phospholipids, reduce matrix effects, and achieve a good recovery while avoiding the added inconvenience of a dry-down step.

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