Sample preparation is crucial in achieving desired LC or GC analytical results. Sample matrix effects can result in an array of interferences, such as lipids, salts, pigments, sugars, proteins, and more which can lead to poor chromatography. When introduced in an analysis, these interferences can cause ion suppression, decreased sensitivity, unreliable results, and can result in increased maintenance and laboratory downtime. The choice of sample preparation technique depends on the nature of the sample, the analytes of interest, and the analytical method being employed.
Phenomenex offers a comprehensive range of products to support multiple sample preparation techniques. Here’s a quick guide to our offerings:
Phenex Syringe Filters and Verex Filter Vials: Ideal for straightforward filtration tasks, ensuring clean samples for your analyses.
Beta-Goneâ„¢: A specialized filter plate method designed to remove beta-glucuronidase from urine samples before drug analysis.
Impactâ„¢: A two-step method using protein precipitation to eliminate harmful proteins, protecting LC columns and detectors. Recommended for plasma or whole blood matrices.
Phreeâ„¢ Phospholipid Removal: For further cleanup of phospholipids and proteins, ensuring optimal sample purity. Best suited for plasma or whole blood samples.
QuEChERS: (Quick, Easy, Cheap, Effective, Rugged, and Safe) A multi-step method for extracting and cleaning up samples from food matrices, particularly effective for multiresidue pesticide analysis using dispersive solid phase extraction (dSPE).
SLE (Simplified/Solid Liquid Extraction): This technique offers a more efficient and easier extraction of analytes into an organic solvent compared to traditional liquid-liquid extraction (LLE). It’s quick and ideal for bioanalytical, food, and clinical research applications that require high sensitivity.
SPE: A widely-used sample preparation technique that isolates and concentrates analytes from complex matrices such as biological fluids, environmental samples, and food products. SPE utilizes a solid adsorbent material packed in cartridges, disks, or well plates to selectively retain target compounds while washing away interfering substances. The main steps include conditioning, equilibration, sample loading, washing, and elution, resulting in clean samples ready for analysis.
Find the perfect solution for your specific sample preparation needs.
