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siRNA Cartridge Purification - Wash #1 Fraction

Sample Preparation Details

Part No:8K-S042-FBR
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 200 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 10 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Intital wash samples

Application Detail (App ID: 15802)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50mM TEAA, pH 7.5 / 5% ACN
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 20 40 60

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Intital wash samples