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Applications

siRNA Cartridge Purification - Wash #1 Fraction

Sample Preparation Details

Condition 1 :1.5 mL 50% Methanol/ Water at 1 mL/min
Condition 2 :2 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min
Load :Load 1.00 mL Sample diluted in Clarity Triophilic 3:1 Buffer
Wash 1 :6 mL Clarity Triophilic 3:1 Buffer, 3 times of at 1 mL/min
Wash 2 :1.5 mL 2% TFA/50mM NaCl, 2 times of at 1 mL/min
Elution 1 :1 mL Methanol / Nuclease-free Water / Acetonitrile (40/40/20) at 1 mL/min
Evaporate: :Evaporate to dryness

Application Detail (App ID: 15802)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50mM TEAA, pH 7.5 / 5% ACN
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 20 40 60

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Intital wash samples