Extraction of Methadone using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MSMS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1 mL of Urine (spiked with analyte and IS) was diluted with 1 mL of buffer(100 mm phosphate buffer, pH 6.0)
Final Prep and Analysis: Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (110 °C)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Methadone 1.5 
2 Methadone-d9 1.5 

Application Detail (App ID: 18651)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 35 65
3 1.01 5 95
4 1.5 5 95
5 1.51 90 10
6 3 90 10

Flow Rate:700 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (110°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available