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Extraction of Methadone using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MSMS

Sample Preparation Details

Condition 1 :1 mL Methanol at 2 mL/min
Condition 2 :1 mL Water at 2 mL/min
Load :Load 1.00 mL Sample diluted in 1
Wash 1 :1 mL 0.1N HCl at 2 mL/min
Wash 2 :1 mL methanol at 2 mL/min
Elution 1 :0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
Elution 2 :0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Initial LC mobile phase (90/10)

Application Detail (App ID: 18651)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 35 65
3 1.01 5 95
4 1.5 5 95
5 1.51 90 10
6 3 90 10

Flow Rate:700 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (110°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Methadone
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2
Methadone-d9
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