Clarity OTX- MS Sensitivity of recovered 21mer at 500ng/mL from plasma

Sample Preparation Details

Condition 1 :0.5 mL MeOH at 1 mL/min
Condition 2 :0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min
Load :Load 500.00 µL Sample diluted in OTX loading buffer/sample
Wash 1 :0.5 mL Clarity OTX Wash Buffer at 1 mL/min
Wash 2 :0.5 mL Clarity OTX Equilibration Buffer at 1 mL/min
Dry :10 um vaccuum
Elution 1 :0.5 mL Clarity OTX Elution Buffer at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Mobile phase A

Application Detail (App ID: 18870)

Part No:00B-4441-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:1%HFIPA/ 0.1%DIEA/ water
Elution B:90% MeOH/ 0.075%HFIPA/ 0.0375% DIEA
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 65 35

Flow Rate:0.25 mL/min
Col. Temp: 60 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

21mer DNA oligo