Clarity OTX- MS Sensitivity of recovered 21mer at 500ng/mL from plasma

Sample Preparation Details

Part No:8E-S103-EGA
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 100 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:10 um vaccuum
Final Prep and Analysis: Inject: 30 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 21mer DNA oligo 500 

Application Detail (App ID: 18870)

Part No:00B-4441-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:1%HFIPA/ 0.1%DIEA/ water
Elution B:90% MeOH/ 0.075%HFIPA/ 0.0375% DIEA
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 65 35

Flow Rate:0.25 mL/min
Col. Temp: 60 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

21mer DNA oligo