Extraction and Analysis of Metronidazole in Facial Cleansers

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1) Dissolve 0.250g of Neutragena Foaming Face Cleaner With Microclear Technology in 10mLs of 0.1N HCL
2) Vortex to achieve homogenous mixture
3) Centrifuge for 5 minutes at 5000g
4) Load
3mL aliquot of sample
Dry:5 min under full vacuum
Final Prep and Analysis: Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (650 °C)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Metronidazole 100 
2 13-C2 15-N-Metronidazole 1000 

Application Detail (App ID: 20631)

Part No:00B-4496-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid
Elution B:MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 2 60 40
3 3 60 40
4 3.1 95 5
5 5 95 5

Flow Rate:300 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (650°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available


13-C2 15-N-Metronidazole