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Applications
Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS
20033
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antiplatyhelmintic Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS
Analytes
1Florfenicol amine
2Sulfanilamide
3Sulfaguanidine
4Tetramisole
5Amoxicillin
6Sulfacetamide
7Lincomycin
8Metronidazole
9Dimetridazole
10Albendazole amino sulfone
11Ronidazole
12Minocycline
13Sulfadiazine
14Thiabendazole
15Sulfathiazole
16Sulfapyridine
17Desethylene ciprofloxacin
18Ractopamine
19Ofloxacin
20Sulfamerazine
21Enoxacin
22Tetracycline
23Norfloxacin
24Ampicillin
25Ciprofloxacin
26Thiamphenicol
27Lomefloxacin
28Enrofloxacin
29Oxytetracycline
30Danofloxacin
31Orbifloxacin
32Difloxacin
33Sarafloxacin
34Sulfamethazine-d4
35Sulfamethazine
362-mercaptobenzimidazole
37Sulfamethizole
38Ipronidazole-OH
39Sulfamethoxypyridazine
40Spiramycin
41Chlorotetracycline
42Sulfachloropyridazine
43Sulfamethoxazole
44Florfenicol
45Sulfadoxine
46Clindamycin
47Tilmicosin
48Sulfisoxazole
49Carbadox
506-phenyl-2-thiouracil
51Albendazole sulfoxide
52Chloramphenicol
53Doxycycline
54Bacitracin
552-Quinoxalinecarboxylic acid
56Oxolinic acid
57Sulfadimethoxine
58Albendazole sulfone
59Erythomycin
60Sulfaquinoloxaline
61Tylosin
62Fenbendazole sulfoxide
63Josamycin
64Fenbendazole sulfone
65Albendazole
66Flumequine
67Sulfanitran
68Ceftiofur
69Mebendazole
70Oxyphenbutazone
71Fenbendazole
72Ketoprofen
73Penicillin G
74Naproxen
75Phenylbutazone
76Flunixin
77Nicarbazin
78Etodolac
79Dipyrone
80Cloxacillin
81Ibuprofen
82Diclofenac
83Indomethacin
84Triclabendazole
85Tolfenamic acid
86Novobiocin
87Niclosamide
88Decoquinate
89Sulfasalazine
90Monensin
91Narasin
92Lasalocid
93Lasalocid
94Rafoxanide
Compound Name
Florfenicol Amine
CID
156406
Molecular Formula
C10H14FNO3S
Molecular Weight
247
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20033)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: Water + 0.1% Formic Acid
B: Methanol + 0.1% Formic Acid
Gradient Profile:
Step No.Time(min)%A%B
10982
27.272080
37.37199
411982
515982
611982
715982
Flow Rate:
0.5 mL/min
Temperature:
40°C
Detection:
Tandem Mass Spec (MS-MS) (600°C)
Detector Info:
SCIEX 4000 QTRAP®
Sample Note:
Sample Prep Steps: 1. Weigh 1 g of homogenized beef kidney sample, kidney juice, or serum into a 50 mL FEP (fluorinated ethylene propylene) tube. Alternatively you can use a disposable polypropylene Corning tube. 2. Add 5 µL of the internal standard work solution. 3. Add 2 mL of water and 8 mL of acetonitrile 4. Mix briefly using a vortex mixer, and then shake for five minutes. 5. Centrifuge at 3450 rcf for five minutes. 6. Decant the supernatant into a 50 mL tube with 500 mg of Sepra C18-E sorbent. 7. Mix briefly using a vortex mixer and shake for 30 seconds. 8. Centrifuge at 3450 rcf for one minute. 9. Place a 5 mL aliquot of the supernatant into a graduated tube. 10. Evaporate down to less than 1 mL. 11. Make up the volume to 1 mL with water. 12. Filter the extract through a 0.45 µm Phenex™ RC syringe filter (part no. AF0-8103-12) and then transfer to a Verex™ autosampler vial (part no. AR0-9925-13). Note: The extracts are now ready for LC/MS/MS analysis. SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

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