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Applications
Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS
20033
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antirheumatic Agents,Antiprotozoal Agents,Antiplatyhelmintic Agents,Antineoplastic Agents,Antinematodal Agents,Antimalarials,Anti-Inflammatory Agents, Non-Steroidal,Anti-Inflammatory Agents,Anti-Infective Agents, Urinary,Anti-Infective Agents, Local
Pharmaceutical
Therapeutic / Clinical

Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS

Analytes
12-mercaptobenzimidazole
22-Quinoxalinecarboxylic acid
36-phenyl-2-thiouracil
4Albendazole
5Albendazole amino sulfone
6Albendazole sulfone
7Albendazole sulfoxide
8Amoxicillin
9Ampicillin
10Bacitracin
11Carbadox
12Ceftiofur
13Chloramphenicol
14Chlorotetracycline
15Ciprofloxacin
16Clindamycin
17Cloxacillin
18Danofloxacin
19Decoquinate
20Desethylene ciprofloxacin
21Diclofenac
22Difloxacin
23Dimetridazole
24Dipyrone
25Doxycycline
26Enoxacin
27Enrofloxacin
28Erythomycin
29Etodolac
30Fenbendazole
31Fenbendazole sulfone
32Fenbendazole sulfoxide
33Florfenicol
34Florfenicol amine
35Flumequine
36Flunixin
37Ibuprofen
38Indomethacin
39Ipronidazole-OH
40Josamycin
41Ketoprofen
42Lasalocid
43Lincomycin
44Lomefloxacin
45Mebendazole
46Metronidazole
47Minocycline
48Monensin
49Naproxen
50Narasin
51Nicarbazin
52Niclosamide
53Norfloxacin
54Novobiocin
55Ofloxacin
56Orbifloxacin
57Oxolinic acid
58Oxyphenbutazone
59Oxytetracycline
60Penicillin G
61Phenylbutazone
62Ractopamine
63Rafoxanide
64Ronidazole
65Sarafloxacin
66Spiramycin
67Sulfacetamide
68Sulfachloropyridazine
69Sulfadiazine
70Sulfadimethoxine
71Sulfadoxine
72Sulfaguanidine
73Sulfamerazine
74Sulfamethazine
75Sulfamethazine-d4
76Sulfamethizole
77Sulfamethoxazole
78Sulfamethoxypyridazine
79Sulfanilamide
80Sulfanitran
81Sulfapyridine
82Sulfaquinoloxaline
83Sulfasalazine
84Sulfathiazole
85Sulfisoxazole
86Tetracycline
87Tetramisole
88Thiabendazole
89Thiamphenicol
90Tilmicosin
91Tolfenamic acid
92Triclabendazole
93Tylosin
Details
LC Conditions (App ID: 20033)
Column:
Brand Name:
Gemini
Part No:
Phase Name:
C18
Sample Note:
Sample Prep Steps: 1. Weigh 1 g of homogenized beef kidney sample, kidney juice, or serum into a 50 mL FEP (fluorinated ethylene propylene) tube. Alternatively you can use a disposable polypropylene Corning tube. 2. Add 5 µL of the internal standard work solution. 3. Add 2 mL of water and 8 mL of acetonitrile 4. Mix briefly using a vortex mixer, and then shake for five minutes. 5. Centrifuge at 3450 rcf for five minutes. 6. Decant the supernatant into a 50 mL tube with 500 mg of Sepra C18-E sorbent. 7. Mix briefly using a vortex mixer and shake for 30 seconds. 8. Centrifuge at 3450 rcf for one minute. 9. Place a 5 mL aliquot of the supernatant into a graduated tube. 10. Evaporate down to less than 1 mL. 11. Make up the volume to 1 mL with water. 12. Filter the extract through a 0.45 µm Phenex™ RC syringe filter (part no. AF0-8103-12) and then transfer to a Verex™ autosampler vial (part no. AR0-9925-13). Note: The extracts are now ready for LC/MS/MS analysis. SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282
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