Reversed Phase Chromatography (RPC)
- Separate Hydrophobic Compounds
Search Our reversed phase HPLC columns and applications
View Featured Reversed Phase HPLC/UHPLC Columns
• Kinetex® Core-Shell HPLC/UHPLC Columns
High efficiency, high performance Core-Shell Technology HPLC/UHPLC columns
• Gemini® HPLC Columns
Organo-silica HPLC columns for extreme pH conditions
• Luna® HPLC Columns
All purpose, ultra-pure pH stable reversed phase HPLC columns
•Synergi™ HPLC Columns
Full range of selectivity for reversed phase method development of challenging mixtures
Learn More about High Pressure Liquid Chromatography (HPLC)
Reversed Phase HPLC/UHPLC Chromatography
Being the most common HPLC/UHPLC separation mode, reversed phase chromatography offers dynamic retention of compounds with hydrophobic and organic functionality. Retention of these compounds by reversed phase involves a combination of hydrophobic and van der Waals type interactions between each target compound and both the stationary phase and mobile phase.
Stationary phases used in reversed phase chromatography typically consist of varying lengths of hydrocarbons such as C18, C8, and C4 or strongly hydrophobic polymers such as styrene divinylbenzene.
- C18 HPLC columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage
- C8 HPLC columns are used when less retention compared to a C18 is needed
- C4 and C5 HPLC columns are commonly used to separate large macromolecules such as proteins
With its large versatility in selectivity, reversed phase HPLC/UHPLC chromatography is incredibly useful for the separation of compounds that comprise a broad range of polarity.
Bring Out the Best In Reversed Phase HPLC/UHPLC Columns
Learn how to maximize reversed phase HPLC column selecitivity and retention under any mobile phase for all target analytes with the FREE Reversed Phase HPLC Column Digital Guide. This guide contains detailed information for proper phase selection and optimizing method development.
Request Your FREE Copy Now »
Need an HPLC column? Start Here.
Learn More About Phenomenex Solutions »