Oligonucleotide Purification

Oligonucleotides are DNA or RNA polymers that are assembled in a step-wise synthesis reaction where each nucleotide is added to the chain. The synthesis typically goes between 18-40 cycles with around 21 mers being the most common length. While the synthesis chemistry is very efficient, each cycle generates little bits of impurity due to the nucleotide not being added to the chain. The result is a mixture of full length sequences and shorter sequences (truncated). Olignoucleotide purification is the process of removing these shorter sequences from the desired full length sequences.

Featured Oligonucleotide Purification Products

Clarity QSP Products for Oligonucleotide Purification

Clarity® QSP™
High-throughput, trityl-on reversed phase cartridge (RPC) purification

Clarity Oligo-RP™
RP-HPLC purification of failure sequences from target sequence

Clarity Oligo-WAX™
Economical, high loading capacity IEX-LC prep-scale purification

Clarity RP-Desalting™
Quick removal of salt and excess reagent

Oligonucleotide Characterization

After purification. synthesized oligonucloetides are often characterized via electrospray mass spectrometry (ES MS) or matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF).

Successful characterization requires the use of products that are highly selective and allow for sensitive detection towards the nucleotide of interest.

Prtoducts for Oligonucleotide Characterization

Featured Oligonucleotide Characterization Products

Clarity® Oligo-RP™
RP-LC/MS analysis with optimized selectivity and sensitivity

Clarity Oligo-MS™
Rapid, high efficiency RP-LC/MS analysis for QC characterization

Clarity OTX™
Extraction of oligo therapeutics from biological samples for LC/MS bioanalysis