Solid phase extraction (SPE) HILIC stationary phase that excels at retention and recovery labeled released N-glycans! Microelution format allows for streamlined processing and clean-up of small sample volumes.
Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.
STEP 1 |
STEP 2 |
STEP 3 |
STEP 4 |
STEP 5 |
Start with Intact Glycoprotein | Deglycosylation Released N-Glycans by PNGase F in buffer solution |
Labeling Glycans 2-AA or 2-AB for accurate and sensitive glycan detection |
Clean-Up Removal of labeling reagent, salts, or surfactants |
Analysis |
96-Well Plate:
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Part No.:
8M-S009-NGA
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Condition:
200 µL Water
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Equilibrate:
200 µL Water/Acetonitrile (15:85)
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Load:
Labeled Sample (up to 400 µL of labeled sample)
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Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
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Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
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Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile
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