Blank Baby Wash using Novum SLE 12cc Tube & Kinetex 5u EVO C18, 100x3.0

Application Detail (App ID: 22924)

Part No:00D-4633-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:10% acetonitrile in water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 4 70 30
3 5 60 40
4 6 60 40

Flow Rate:1.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:Novum SLE 12cc Tube
Novum SLE 12cc Tube

Extraction Procedure
Sample pre-treatment
0.5 ± 0.05 g spiked Aveeno Baby (Wash & Shampoo) sample
Add 1.25 mL acetone
Add 0.6 ml 1% Nacl solution
Vortex for 30 secs
Wait 30 secs till the emulsion dissipates
Centrifuge for 10 mins at 3000 rpm
Collect the supernatant and proceed to load
Sample loading
"Load the sample from pre-treatment step above onto the Novum SLE cartridge and apply a short and gentle pulse of vacuum (~ 5" of Hg for 5-10 sec or until the sample has completely entered the media).
"Wait for 5 minutes.
"Dispense 10 mL of ethylacetate onto the SLE media and allow the solvent to elute by gravity for 10 mins and collect the eluant.
"Apply vacuum at 5"-10"of Hg to complete the extraction.

NOTE: Prolonged application of vacuum will result in elution of body wash out of the SLE media and into the final extracted solvent.

Dry down
"Evaporate the final extract to complete dryness under slow stream of N2 at 45°C

"Reconstitute the dry residue in 0.5 mL of methanol
"Centrifuge for 5 mins at 6000 rpm
"Collect supernatant to shoot on HPLC

Analyte Details

Analyte Detail not available