Product Information
Phase Detail
Biozen Glycan<br class="t-last-br" />
Biozen Glycan 2.6 µm core-shell HPLC columns provide optimized separation of released and labelled glycans.
Biozen Glycan
Biozen Glycan
The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems, can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities, to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the Biozen Glycan excels with increased polar retention and selectivity.
Separation Mode HILIC |
Pore Size 100 |
Particle Size 2.6 |
Recommended Use High efficiency and selectivity for released glycans |
Minimize your priming with our BioTi™ Biocompatible hardware
Biozen Titanium BioTi Hardware
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Columns:
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Dimension:
150 x 4.6 mm
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Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0
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Flow Rate:
0.3 mL/min
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Temperature:
Ambient
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Detection:
UV @ 280 nm
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Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL |
Conditions for both separations:
Column:
Biozen 2.6 µm Glycan
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4773-AN |
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Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5 B: Acetonitrile |
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Gradient:
Time (min)
0
10 24 38.5 38.6 40.6 40.7 48 | % B
78 74.5 72 55.9 40 40 78 78 |
Flow Rate:
0.5 mL/min |
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Temperature:
50 °C |
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Detection:
FLD ex/em 285/345 nm |
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Samples:
As noted |
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Sample Preparation Solutions
bioZen Glycan Batch-to-Batch Reproducibility
Conditions for all separations:
Column:
bioZen 2.6 μm Glycan
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Dimension:
100 x 2.1 mm
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Part No.:
00D-4773-AN |
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Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5 B: Acetonitrile |
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Gradient:
Time (min)
0
16 25.9 27.2 27.3 30 | % B
76 72 40 40 76 76 |
Flow Rate:
0.5 mL/min |
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Temperature:
50 °C |
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Detection:
FLD ex/em 330/420 nm |
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Samples:
Human IgG Glycan Library |
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2-AB Labeled Bovine Fetuine Sialylated Glycans
Column:
bioZen 2.6 μm Glycan
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Dimension:
100 x 2.1 mm
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Part No.:
00D-4773-AN |
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Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5 B: Acetonitrile |
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Gradient:
Time (min)
0
20 24 24.1 30 | % B
60 20 20 60 60 |
Flow Rate:
0.5 mL/min |
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Temperature:
80 °C |
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Detection:
FLD ex/em 330/420 nm |
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Sample:
Bovine Fetuin |
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Glycan Analysis: HILIC
Column:
bioZen 2.6 μm Glycan
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Dimension:
100 x 2.1 mm
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Part No.:
00D-4773-AN |
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Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5 B: Acetonitrile |
|
Gradient:
Time (min)
0
16 25.9 27.2 27.3 30 | % B
76 72 40 40 76 76 |
Flow Rate:
As Noted |
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Temperature:
50 °C |
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Detection:
FLD ex/em 330/420 nm |
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Sample:
Human IgG Glycan Library |
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