Product Information
Phase Detail
Clarity RP-Desalting
Clarity RP Desalting tubes allow for rapid desalting of trityl off synthetic oligo mixtures
Clarity RP-Desalting
Clarity RP-Desalting
Quick Salt Removal
Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.
- ~70 % purity and ~80 % recovery of target oligo
- Economical and disposable format
U.S. Patent No. 7,119,145
Brand Clarity (SPE) |
Recommended Use Eliminate salts and reagents from trityl-off synthetic oligo mixtures resulting in high recoveries |
General Extraction
Desalting of Dye-Labeled DNA
A quencher-labeled sample of DNA (25nt) with the sequence FAMTTGACTTAGACTTAGA-CTTAGTTT was desalted using Clarity RP Desalting tubes in the 200 mg/3 mL format. Collection fractions were then analyzed for purity and recovery.
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Column:
Clarity 3 µm Oligo-RP C18
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Dimension:
50 x 4.6 mm
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Part No.:
00B-4441-E0
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Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile
B: Methanol |
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Gradient:
A/B (90:10) to A/B (40:60) in 20 min
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Flow Rate:
1 mL/ min
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Detection:
UV @ 260 nm
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Sample:
25nt DNA oligonucleotide
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Column:
Clarity 3 µm Oligo-RP C18
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Dimension:
50 x 4.6 mm
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Part No.:
00B-4441-E0
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Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile
B: Methanol |
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Gradient:
A/B (90:10) to A/B (40:60) in 20 min
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Flow Rate:
1 mL/ min
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Detection:
UV @ 260 nm
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Sample:
40nt DNA
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