Region

Oligonucleotide

Purification Products

Clarity (SPE) Sample Preparation Information


Isolation and Purification of Synthetic Oligonucleotides

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

  • Works for desalting and clean-up of biological samples
  • Specially formulated buffers solutions available for optimal performance
  • Quick protocols for rapid results

Clarity BioSolutions Product Selection

Purification

  Clarity QSP™ Clarity Oligo-RP™
Clarity Oligo-XT
Clarity Oligo-SAX™ Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
purification
RP-HPLC purification of failure
sequences from target sequences
Economical, high loading capacity
IEX-LC prep-scale purification
Quick removal or salt & excess reagent
Purities >90% >90% >90% ~70%
Recoveries ~90% ~70% >90% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

  Clarity Oligo-RP™ Clarity Oligo-MS™
Clarity Oligo-XT
Clarity Oligo-OTX™
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Material Characteristics

Clarity Products Particle
Support
Bonded
Phase
Particle
shape/Size
(µm)
Pore Size
(Å)
Surface
Area
(m2/g)
Clarity QSP™ Cartridges Polymer (PSDVB) Hydrophilic polymer coating 35 500 300
Clarity Oligo-RP™
LC Columns
TWIN
(silica, organic, composite)
C18 3, 5, 10 110 375
Clarity Oligo-WAX™
LC Columns
Silica Crosslinked polyamine (WAX) 10 360
Clarity RP-Desalting™ Tubes Silica C18 55 140 300
Clarity Oligo-MS™
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200*
(*effective)
Clarity OTX™
Extraction Plates
Polymer (surface
modified PSDVB)
Mixed-mode anion exchanger 33 85 800
Clarity Oligo-SAX
LC Columns
Polymer (surface
modified PSDVB)
Hydrophilic quaternary amine 5
Clarity Oligo-XT
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200

RP Desalting Phase Detail


RP Desalting Phase Information


Quick Salt Removal

Clarity RP-Desalting™ tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.

  • ~70 % purity and ~80 % recovery of target oligo
  • Economical and disposable format

Desalting of Dye-Labeled DNA

A quencher-labeled sample of DNA (25nt) with the sequence FAMTTGACTTAGACTTAGA-CTTAGTTT was desalted using Clarity RP-Desalting tubes in the 200 mg/3 mL format. Collection fractions were then analyzed for purity and recovery using the above protocol.

Document Type:
Document Type Title
Technical Notes Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High Resolution Mass Spectrometry (TN-0097) Add to My Documents
Brochures Clarity Biosolutions for Synthetic DNA/RNA Add to My Documents
Brochures Clarity QSP Add to My Documents
MSDS AL0-8280_Clarity QSP DNA Buffer SDS Add to My Documents
MSDS AL0-8282_Clarity QSP RNA Buffer SDS Add to My Documents
MSDS AL0-8579_Clarity OTX Lysis Loading Buffer SDS Add to My Documents
Technical Notes Avoiding Depurination During Trityl-on Purification (TN-0008) Add to My Documents
Brochures Clarity OTX Add to My Documents
Supporting Docs Clarity OTX User Guide Add to My Documents
Supporting Docs Clarity QSP User’s Guide (DNA) Add to My Documents
Supporting Docs Clarity QSP User’s Guide (RNA) Add to My Documents
Technical Notes Trityl-on RNA and DNA Purification (TN-0009) Add to My Documents
MSDS KS0-8494_Clarity OTX Elution Buffer SDS (for kit) Add to My Documents
MSDS KS0-8494_Clarity OTX Equilibration Buffer SDS (for kit) Add to My Documents
MSDS KS0-8494_Clarity OTX Wash Buffer SDS Add to My Documents
MSDS KS0-8494_Clarity OTX Wash Buffer SDS (for kit) Add to My Documents
Technical Notes Oligonucleotide Therapeutics from Biological Matrices (TN-0048) Add to My Documents
Supporting Docs Clarity Overview Flyer Add to My Documents
Supporting Docs Collection Plate and Sealing Mat Selection Guide Add to My Documents
Other Protein and Peptide Users Guide Add to My Documents
Technical Notes RNA Sample Preparation and High Throughput Purification for TBDMS and TOM Chemistries (TN-0016) Add to My Documents
Supporting Docs SPE 10, 12, and 24 Position Vacuum Manifold Instructions Add to My Documents
Supporting Docs SPE 96-Well Vacuum Manifold Add to My Documents
Phase: Sorbent Mass:
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Part Number:8B-S041-FBJ

Description:Clarity® RP Desalting, 200 mg / 3 mL, Tubes , 50/Pk

Sorbent Type:Silica-based

Format:Tube

Phase:C18 / ODS

Target Analytes:Trityl-off DNA and RNA sequences


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