Polar Probes on Synergi Hydro

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4375-E0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: Phosphate Buffer, pH 2.5

B: Acetonitrile

C: 0

D: 0

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

5

100

0

3

5.01

95

5

4

10

70

30

5

10.5

100

0

6

14

100

0

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282