Phencyclidine from urine using Strata-X-Drug B and Kinetex C18, 50 x 2.1 mm by LCMS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: Methanol with 0.1% Formic Acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

1

5

95

3

2

5

95

4

2.1

75

25

5

4

75

25

Flow Rate

400.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (500°C)

Detection Info

Sample Preparation Details

Description

Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S128-UCH

Pretreatment Note

Evaporate eluant to dryness and reconstitute in 1 mL of 20% methanol in water. Phencyclidine was spiked into the urine at the 25ng/mL cutoff.

Condition 1

2 at 2

Condition 2

2 at 2

Load

Load 4.00 mL Sample diluted in Pre-treated Sample

Wash 1

2mL of 100mM Sodium acetate buffer (pH 5.0)

Wash 2

2mL of Methanol

Dry

10min @ >10 in Hg

Elution 1

2mL of Ethyl Acetate: IPA:NH4OH (7:2:1)

Evaporation

Evaporate to dryness

Reconstitute

1 mL 20% methanol in water