6-MAM by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

1

30

70

3

3

30

70

4

3

30

70

Flow Rate

400.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S128-UCH

Pretreatment Note

Evaporate eluant to dryness and reconstitute in 1mL of 10% methanol in formic acid (0.1%)

Load

Load 4.00 mL Sample diluted in 100mM Phosphate buffer

Wash 1

2mL of 100mM sodium acetate buffer pH 5.0

Wash 2

2mL of Methanol

Dry

10 min at 10 inches of Hg

Elution 1

2mL of Ethyl acetate: IPA:NH4OH (7:2:1)

Evaporation

Evaporate to dryness

Reconstitute

1000 µL 10% methanol in formic acid (0.1%)