LC/MS/MS Analysis of Ethyl Glucuronide and Ethyl Sulfate on Gemini NX-C18

LC Conditions

Column

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea

Brand

Gemini

Part No

00D-4453-B0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 5 mM Ammonium Formate

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

1

54

46

3

1.01

20

80

4

2

0

100

Flow Rate

300.00 mL/min

Temperature

25°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-4286 - Holder Part No.: KJ0-4282