LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: 0.1% Formic acid in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

2

3.5

0

100

3

3.51

60

40

4

6.5

60

40

Flow Rate

400.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S128-UCH

Pretreatment Note

Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 40% acetonitrile.

Load

Load Sample

Wash 1

2 mLs of 100mM sodium acetate buffer (pH 5)

Wash 2

2 mLs of Methanol

Dry

10 minutes @ full vacuum

Elution 1

2mLs of Ethyl acetateNH4OH (7:2:1)

Evaporation

Evaporate to dryness

Reconstitute

1 mL 40% acetonitrile in water