LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 5mM Ammonium acetate

B: 5mM Ammonium acetate in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

5.5

0

100

3

6.5

0

100

4

6.51

85

15

5

9

85

15

Flow Rate

500.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S128-UCH

Pretreatment Note

Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 15% methanol.

Load

Load Sample

Wash 1

2mLs of 100mM Sodium acetate (pH 5.0) buffer

Wash 2

2mLs of Methanol

Dry

10 minutes @ full vacuum

Elution 1

2mLs of Ethyl acetateNH4OH (7:2:1)

Evaporation

Evaporate to dryness

Reconstitute

1 mL 15% methanol in water