EPA 539 on Gemini NX-C18 3 um 100 X 2.0 mm

LC Conditions

Column

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea

Brand

Gemini

Part No

00D-4453-B0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 0.2%NH4OH in water

B: 0.2%NH4OH in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

0.01

65

35

3

0.6

35

65

4

7.5

35

65

5

8.5

15

85

6

13

15

85

7

13.01

65

35

8

15

65

35

Flow Rate

0.20 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info