Multivitamin Mix through a Nylon Filter Vial on a Synergi 4um Hydro-RP 150x4.6mm

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4375-E0

Phase Name

Hydro-RP

Guard Cartridge:

AJ0-4287

Guard Holder:

KJ0-4282

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium Phosphate + 0.1% Sodium-1-Hexanesulfonate

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

3

97

3

3

18

50

50

4

18.1

97

3

5

20

97

3

Flow Rate

1.50 mL/min

Temperature

30°C

System

Agilent Technologies 1100 Series Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 210 nm (nanometers)

Detection Info