Water-soluble vitamins standard on Synergi Hydro-RP

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4375-E0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 20mM KH2PO4 + 0.1% Hexane sulfonate, pH 3.0

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

3

97

3

3

18

50

50

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7511 - Holder Part No.: KJ0-4282