| 1Poly dT 12-18 Oligonucleotide |
|---|
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Applications
Poly dT 12-18 standard on Oligo-RP (modifying TEA/HFIP Ratio)
18089
Separation Mode: Reversed Phase
Reversed Phase
Oligo-RP
HPLC
Life Science
Pharmaceutical/Biopharmaceutical
Oligonucleotide
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 18089)
Column: |
Brand Name: Clarity |
Part No: |
Phase Name: Oligo-RP |
Sample Note: Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture. |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.