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Application Focus: To show the calibration curves for the three different BioSep-SEC phases
For this application, calibration curves are generated for the three different BioSep-SEC phases using standard proteins of defined molecular weights and non-denaturing operating conditions (100 mM phosphate, 300 mM NaCl, pH 7.0, 1mL/min). For each media (300 x 7.8 mm column) the elution volume where each protein peak elutes was plotted against the log of the expected molecular weight of each protein. A 3rd order polynomial is used to plot the calibration curve for each phase.
Specific applications used to generate curves were: App ID# 18892 for BioSep2000, App ID# 18928 for BioSep3000 and App ID#18936 for BioSep4000. While a polynomial was used to generate calibration curves in this example, one could use a linear plot if one removes the data points at the ends of the molecular weight ranges.
1. 11 Proteins
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