| 1THC-OH |
|---|
| 2THC-OH-d3 |
| 3THC-COOH |
| 4THC-COOH-d3 |
| 5THC |
| 6THC-d3 |
Compound Name Hexestrol Dibutyrate |
CID 644022 |
Molecular Formula C21H30O3 |
Molecular Weight 330 |
No. Hydrogen Bond Acceptors 3 |
No. Hydrogen Bond Donors 2 |
Applications
THC and Metabolites from Whole Blood on Strata-X-Drug B and Kinetex C18 by LC-MS-MS
19947
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Psychotropic Drugs
Forensics/Toxicology
Chemical Substance
Therapeutic / Clinical
Applications
THC and Metabolites from Whole Blood on Strata-X-Drug B and Kinetex C18 by LC-MS-MS
Analytes
Details
LC Conditions (App ID: 19947)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 1 mM ammonium formate with 0.1% FA B: 0.1% FA/methanol:acetonitrile(1:1) | ||||||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||||||
Flow Rate: 0.4 mL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||||||
Detector Info: SCIEX API 5000™ | ||||||||||||||||||||||||
Sample Note: Mass spec conditions:
Polarity Analyte Q1 Q3 DP CE
(+) THC-OH (*) 331.0 193.3 55 31
(+) THC-OH 331.0 201.2 55 33
(+) THC-OH 331.0 175.3 55 31
(+) THC-OH-d3 334.0 196.3 55 31
(+) THC (*) 315.2 193.1 60 31
(+) THC 315.2 259.1 60 35
(+) THC-d3 318.2 196.1 60 25
(+) THC-COOH (*) 345.2 327.0 55 29
(+) THC-COOH 345.2 299.2 55 49
(+) THC-COOH-d3 348.0 330.1 55 29
CAD: 7 GS1: 60 GS2: 45 TEM: 600 CUR: 25 IS: 5500
* indicates quant ion |
Sample Preparation Details
Description: Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk |
Part Number: |
Pre Treatment Note: Evaporate eluant to dryness under 50 °C nitrogen. Reconstitute in 500uL of initial mobile phase.
Extraction was automated and performed using a Gilson GX-274 ASPEC system |
Sample PreTreatment: Evaporate eluant to dryness under 50 °C nitrogen. Reconstitute in 500uL of initial mobile phase.
Extraction was automated and performed using a Gilson GX-274 ASPEC system |
Wash 1: 2mL of ACN:Water (15:85) |
Evaporation: Evaporate to dryness |
1 Shake 2mL of Ethyl acetate:Isopropanol (85:15) |
1 Wash 2mL of ACN:Water (15:85) |
Order Items Used in This Application
00B-4462-AN
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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