Analysis of crude peptide mixture on Kinetex 2.6u XB-C18 150x4.6mm

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4496-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

10

5

95

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info