Antihistamines on Kinetex 2.6u F5 50x4.6mm at pH 2.9 and pH 7.2

LC Conditions

Column

Kinetex 2.6 µm F5 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4723-E0

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium Phosphate pH 2.9

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

5

10

90

3

6

10

90

4

7

70

30

5

10

70

30

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info