1C8:0 |
---|
2C10:0 |
3C11:0 (I.S.) |
4C12:0 |
5C14:0 |
6C16:0 |
7C18:0 |
8C18:1 cis 9 |
9C18:2 cis 9,12 |
10C18:3 cis 9,12,15 |
11C20:0 |
12C20:1 cis 11 |
13C20:4 cis 5,8,11,14 |
14C22:0 |
15C24:0 |
16C22:6 cis 4,7,10,13,16,19 |
1C8:0 |
---|
2C10:0 |
3C11:0 (I.S.) |
4C12:0 |
5C14:0 |
6C16:0 |
7C18:0 |
8C18:1 cis 9 |
9C18:2 cis 9,12 |
10C18:3 cis 9,12,15 |
11C20:0 |
12C20:1 cis 11 |
13C20:4 cis 5,8,11,14 |
14C22:0 |
15C24:0 |
16C22:6 cis 4,7,10,13,16,19 |
Column: |
Part No: |
Phase Name: ZB-FAME |
Sample Notes: Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh out 500 mg of powdered infant formula into a scintillation vial
2. Dissolve (or dilute) sample in 5 mL toluene
3. Add 6 mL 10 % acetyl chloride solution in methanol
4. Incubate @ 80 °C for 2 hours
5. After incubation add 10 mL 10 % Na2CO3 solution and centrifuge at 5000 rpm for 5 min
6. Extract organic layer for GC analysis |